REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION

蛋白质和核酸之间的特异性相互作用是 影响表达、复制和包装的基本事件 遗传因素 该提案描述了分子方面的研究 这样的互动。 三个系统正在调查中。 a.我们建议研究参与产生的机制， 正痘病毒基因组的自发重排， 特别是决定范围、地点和 这种重组的频率。 我们还将研究控制病毒 通过鉴定和表征强晚期启动子的转录 牛痘病毒基因组中的元件。 我们还将尝试识别 牛痘病毒DNA的复制起点。 B。我们将继续对37Kd复制的突变体进行研究 质粒pSC101的起始蛋白;我们将测试这些突变体的 他们的复制能力 我们还将在E.大肠杆菌，并纯化 HTLV-1的pX蛋白和El开放阅读框编码的蛋白 关于BPV 为此，我们最近将使用一种表达载体 构建的胶原蛋白载体pJG200。 我们还将测试 这些蛋白质具有序列特异性DNA结合能力。 C.我们将研究参与的机制， 抑制小核的加工和组装 水泡性口炎病毒感染细胞中的核糖核蛋白。 这 效应与U1和U2前体形式的积累有关 核糖核酸 我们将确定信使RNA的剪接是否一般是 也受到抑制。 我们亦会继续研究如何识别 与核糖体RNA相关的蛋白质的功能，并确定 编码这些蛋白质的基因的基因组组织。 最后我们 将对La蛋白基因进行测序;这是VSV与之结合的蛋白质。 前导RNA在感染细胞中相互作用。 我们将绘制内含子和外显子 以及确定启动子和其他上游控制 它的mRNA序列。

Specific interactions between proteins and nucleic acids are the fundamental events that effect the expression, replication and packaging of genetic elements. This proposal describes studies on the molecular aspects of such interactions. Three systems are under investigation. A. We propose to examine the mechanisms involved in the generation of spontaneous rearrangements of the orthopoxvirus genome, focusing in particular on the factors that determine the extent, the sites and the frequency of such rearrangements. We will also study the control of viral transcription by identifying and characterizing strong late promoter elements in the cowpox virus genome. We will also attempt to identify the replication origins in cowpox virus DNA. B. We will continue our studies on mutants of the 37 Kd replication initiator protein of the plasmid pSC101; we will test these mutants for their ability to replicate. We will also express in E. coli and purify the pX protein of HTLV-1 and the protein encoded by the El open reading frame of BPV. We will use for this purpose an expression vector recently constructed by us, namely the collagen vector pJG200. We will also test these proteins for sequence specific DNA-binding ability. C. We will study the mechanisms involved in the rapid and complete inhibition of the processing and assembly of small nuclear ribonucleoproteins in cells infected with vesicular stomatitis virus. This effect is associated with the accumulation of precursor forms of U1 and U2 RNA. We will determine whether splicing of messenger RNAs in general is also inhibited. We will also continue our studies on the identification and function of proteins associated with ribosomal RNA and identify the genomic organization of the genes encoding these proteins. Finally, we will sequence the La protein gene; this is the protein with which VSV leader RNA interacts in infected cells. We will map the introns and exons of this gene, as well as identify the promoters and other upstream control sequences of its mRNA.