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REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION

蛋白质核酸相互作用的调节功能

基本信息

批准号：
3093399
负责人：
Wolfgang K Joklik
金额：
$ 90.61万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1981
资助国家：
美国
起止时间：
1981-08-01 至 1993-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3093399
关键词：
DNA binding protein RNA biosynthesis binding proteins protein biosynthesis virus genetics virus replication virus virus interaction

项目摘要

Specific interactions between proteins and nucleic acids are the fundamental events that effect the expression, replication and packaging of genetic elements. This proposal describes studies on the molecular aspects of such interactions. Three systems are under investigation. A. We propose to examine the mechanisms involved in the generation of spontaneous rearrangements of the orthopoxvirus genome, focusing in particular on the factors that determine the extent, the sites and the frequency of such rearrangements. We will also study the control of viral transcription by identifying and characterizing strong late promoter elements in the cowpox virus genome. We will also attempt to identify the replication origins in cowpox virus DNA. B. We will continue our studies on mutants of the 37 Kd replication initiator protein of the plasmid pSC101; we will test these mutants for their ability to replicate. We will also express in E. coli and purify the pX protein of HTLV-1 and the protein encoded by the El open reading frame of BPV. We will use for this purpose an expression vector recently constructed by us, namely the collagen vector pJG200. We will also test these proteins for sequence specific DNA-binding ability. C. We will study the mechanisms involved in the rapid and complete inhibition of the processing and assembly of small nuclear ribonucleoproteins in cells infected with vesicular stomatitis virus. This effect is associated with the accumulation of precursor forms of U1 and U2 RNA. We will determine whether splicing of messenger RNAs in general is also inhibited. We will also continue our studies on the identification and function of proteins associated with ribosomal RNA and identify the genomic organization of the genes encoding these proteins. Finally, we will sequence the La protein gene; this is the protein with which VSV leader RNA interacts in infected cells. We will map the introns and exons of this gene, as well as identify the promoters and other upstream control sequences of its mRNA.

蛋白质和核酸之间的特殊相互作用是

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transcription of orthopoxvirus telomeres at late times during infection.

感染后期正痘病毒端粒的转录。

DOI：
10.1016/0042-6822(90)90187-v
发表时间：
1990
期刊：
Virology
影响因子：
3.7
作者：
Parsons,BL;Pickup,DJ
通讯作者：
Pickup,DJ

Transcriptional activation of a conserved sequence element by ras requires a nuclear factor distinct from c-fos or c-jun.

ras 对保守序列元件的转录激活需要不同于 c-fos 或 c-jun 的核因子。

DOI：
10.1073/pnas.87.10.3866
发表时间：
1990
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
Owen,RD;Ostrowski,MC
通讯作者：
Ostrowski,MC

Transcription of the terminal loop region of vaccinia virus DNA is initiated from the telomere sequences directing DNA resolution.

痘苗病毒 DNA 末端环区域的转录是从指导 DNA 解析的端粒序列开始的。