REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
批准号:
3093399
负责人:
Wolfgang K Joklik
金额:
$90.61万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1981
资助国家:
美国
项目状态:
已结题
起止时间:
1981-08-01 至 1993-07-31
中文摘要
蛋白质和核酸之间的特殊相互作用是
英文摘要
Specific interactions between proteins and nucleic acids are the
fundamental events that effect the expression, replication and packaging of
genetic elements. This proposal describes studies on the molecular aspects
of such interactions. Three systems are under investigation.
A. We propose to examine the mechanisms involved in the generation of
spontaneous rearrangements of the orthopoxvirus genome, focusing in
particular on the factors that determine the extent, the sites and the
frequency of such rearrangements. We will also study the control of viral
transcription by identifying and characterizing strong late promoter
elements in the cowpox virus genome. We will also attempt to identify the
replication origins in cowpox virus DNA.
B. We will continue our studies on mutants of the 37 Kd replication
initiator protein of the plasmid pSC101; we will test these mutants for
their ability to replicate. We will also express in E. coli and purify the
pX protein of HTLV-1 and the protein encoded by the El open reading frame
of BPV. We will use for this purpose an expression vector recently
constructed by us, namely the collagen vector pJG200. We will also test
these proteins for sequence specific DNA-binding ability.
C. We will study the mechanisms involved in the rapid and complete
inhibition of the processing and assembly of small nuclear
ribonucleoproteins in cells infected with vesicular stomatitis virus. This
effect is associated with the accumulation of precursor forms of U1 and U2
RNA. We will determine whether splicing of messenger RNAs in general is
also inhibited. We will also continue our studies on the identification
and function of proteins associated with ribosomal RNA and identify the
genomic organization of the genes encoding these proteins. Finally, we
will sequence the La protein gene; this is the protein with which VSV
leader RNA interacts in infected cells. We will map the introns and exons
of this gene, as well as identify the promoters and other upstream control
sequences of its mRNA.
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DOI:
10.1016/0042-6822(90)90187-v
发表时间:
1990
期刊:
Virology
影响因子:
3.7
作者:
[Parsons,BL, Pickup,DJ]
通讯作者:
Pickup,DJ
Transcriptional activation of a conserved sequence element by ras requires a nuclear factor distinct from c-fos or c-jun.
ras 对保守序列元件的转录激活需要不同于 c-fos 或 c-jun 的核因子。
DOI:
10.1073/pnas.87.10.3866
发表时间:
1990
期刊:
Proceedings of the National Academy of Sciences of the United States of America
影响因子:
11.1
作者:
[Owen,RD, Ostrowski,MC]
通讯作者:
Ostrowski,MC
Transcription of the terminal loop region of vaccinia virus DNA is initiated from the telomere sequences directing DNA resolution.
痘苗病毒 DNA 末端环区域的转录是从指导 DNA 解析的端粒序列开始的。
DOI:
10.1016/0042-6822(91)90905-q
发表时间:
1991
期刊:
Virology
影响因子:
3.7
作者:
[Hu,FQ, Pickup,DJ]
通讯作者:
Pickup,DJ
A nuclear factor that binds to ras-responsive enhancer elements is present in human tumor cells.
人类肿瘤细胞中存在与 ras 反应增强子元件结合的核因子。
DOI:
--
发表时间:
1990
期刊:
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子:
--
作者:
[Owen,RD, Ostrowski,MC]
通讯作者:
Ostrowski,MC
Primary structure of the essential replicon of the plasmid pSC101.
质粒 pSC101 必需复制子的一级结构。
DOI:
10.1073/pnas.80.21.6557
发表时间:
1983
期刊:
Proceedings of the National Academy of Sciences of the United States of America
影响因子:
11.1
作者:
[Vocke,C, Bastia,D]
通讯作者:
Bastia,D
共 11 条
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093396
-
项目类别:
-
资助金额:$90.43万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093392
-
项目类别:
-
资助金额:$102.4万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093397
-
项目类别:
-
资助金额:$88.96万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093398
-
项目类别:
-
资助金额:$85.19万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093394
-
项目类别:
-
资助金额:$62.36万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
REGULATORY FUNCTIONS OF PROTEIN NUCLEIC ACID INTERACTION
-
批准号:3093395
-
项目类别:
-
资助金额:$62.83万
-
财政年份:1981
-
负责人:Wolfgang K Joklik
-
依托单位:
VIRAL ONCOLOGY
-
批准号:3532479
-
项目类别:
-
资助金额:$27.92万
-
财政年份:1980
-
负责人:Wolfgang K Joklik
-
依托单位:
VIRAL ONCOLOGY
-
批准号:3532477
-
项目类别:
-
资助金额:$25.66万
-
财政年份:1980
-
负责人:Wolfgang K Joklik
-
依托单位:
VIRAL ONCOLOGY
-
批准号:3532480
-
项目类别:
-
资助金额:$24.66万
-
财政年份:1980
-
负责人:Wolfgang K Joklik
-
依托单位:
VIRAL ONCOLOGY
-
批准号:3532478
-
项目类别:
-
资助金额:$28.83万
-
财政年份:1980
-
负责人:Wolfgang K Joklik
-
依托单位:
VIRAL ONCOLOGY
-
批准号:3532474
-
项目类别:
-
资助金额:$19.1万
-
财政年份:1980
-
负责人:Wolfgang K Joklik
-
依托单位:
BASIC MECHANISMS IN INFECTIOUS DISEASES
-
批准号:3530877
-
项目类别:
-
资助金额:$15.16万
-
财政年份:1978
-
负责人:Wolfgang K Joklik
-
依托单位:
BASIC MECHANISMS IN INFECTIOUS DISEASES
-
批准号:3530876
-
项目类别:
-
资助金额:$12.15万
-
财政年份:1978
-
负责人:Wolfgang K Joklik
-
依托单位:
BASIC MECHANISMS IN INFECTIOUS DISEASES
-
批准号:3530878
-
项目类别:
-
资助金额:$16.2万
-
财政年份:1978
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:3124482
-
项目类别:
-
资助金额:$30.39万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:2058880
-
项目类别:
-
资助金额:$30.4万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:3124477
-
项目类别:
-
资助金额:$31.62万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:3124476
-
项目类别:
-
资助金额:$34.29万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:3124479
-
项目类别:
-
资助金额:$37.15万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
MACROMOLECULAR SYNTHESIS IN VIRUS-INFECTED CELLS
-
批准号:3124480
-
项目类别:
-
资助金额:$28.09万
-
财政年份:1974
-
负责人:Wolfgang K Joklik
-
依托单位:
海外基金