Moonlighting functions of endocytic proteins during the cell cycle

细胞周期中内吞蛋白的兼职功能

• 批准号: BB/H015582/2
• 负责人: Stephen Royle
• 金额: $ 9.45万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH015582%2F2
• 关键词: Moonlighting; functions; endocytic; proteins; during

Cells can be thought of as islands - they are closed to the outside world because they have a plasma membrane that doesn't let anything through. However, cells have evolved tiny portals for things to enter in a tightly regulated way. These portals are working all the time, but they are shut down when the cell needs to divide. It is important that no mistakes are made at this time. Otherwise the cell could die or it could start to grow in an unusual way leading to disease e.g. cancer. Nobody knows how this shutdown happens. We want to study the portals using a machine that can tell us all the pieces that make up a portal. If we compare portals from normal cells with those that are dividing this will tell us which pieces are missing in dividing cells. We can then find out how the cell takes these pieces away at the start of cell division. We think that this work is important to do. These portals are hijacked by viruses: they use portals as a way to infect cells. If we knew how the cell shuts down the portals, we could think of ways to switch them off using drugs. These drugs could be used as anti-viral treatments.

细胞可以被认为是岛屿--它们与外界封闭，因为它们有一个不允许任何东西通过的质膜。然而，细胞已经进化出了微小的门户，让事物以一种严格调控的方式进入。这些入口一直在工作，但当细胞需要分裂时，它们就会关闭。重要的是在这个时候不犯任何错误。否则，细胞可能会死亡，或者开始以不寻常的方式生长，导致疾病，例如癌症。没有人知道这种关闭是如何发生的。我们想用一台能告诉我们组成传送门的所有部件的机器来研究传送门。如果我们比较正常细胞和正在分裂的细胞的入口，这将告诉我们哪些片段在分裂的细胞中缺失。然后我们就可以知道细胞是如何在细胞分裂开始时将这些碎片带走的。我们认为这项工作很重要。这些门户网站被病毒劫持：它们将门户网站作为感染细胞的一种方式。如果我们知道细胞是如何关闭这些通道的，我们就可以想办法用药物来关闭它们。这些药物可以用作抗病毒治疗。

项目成果

Mitotic inhibition of clathrin-mediated endocytosis.

• DOI: 10.1007/s00018-012-1250-8
• 发表时间: 2013-09
• 期刊: CELLULAR AND MOLECULAR LIFE SCIENCES
• 影响因子: 8
• 作者: Fielding, Andrew B.;Royle, Stephen J.
• 通讯作者: Royle, Stephen J.

An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.

• DOI: 10.7554/elife.00829
• 发表时间: 2014-02-18
• 期刊: eLife
• 影响因子: 7.7
• 作者: Kaur S;Fielding AB;Gassner G;Carter NJ;Royle SJ
• 通讯作者: Royle SJ