Moonlighting functions of endocytic proteins during the cell cycle

细胞周期中内吞蛋白的兼职功能

• 批准号: BB/H015582/1
• 负责人: Stephen Royle
• 金额: $ 23.84万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH015582%2F1
• 关键词: Moonlighting; functions; endocytic; proteins; during

Cells can be thought of as islands - they are closed to the outside world because they have a plasma membrane that doesn't let anything through. However, cells have evolved tiny portals for things to enter in a tightly regulated way. These portals are working all the time, but they are shut down when the cell needs to divide. It is important that no mistakes are made at this time. Otherwise the cell could die or it could start to grow in an unusual way leading to disease e.g. cancer. Nobody knows how this shutdown happens. We want to study the portals using a machine that can tell us all the pieces that make up a portal. If we compare portals from normal cells with those that are dividing this will tell us which pieces are missing in dividing cells. We can then find out how the cell takes these pieces away at the start of cell division. We think that this work is important to do. These portals are hijacked by viruses: they use portals as a way to infect cells. If we knew how the cell shuts down the portals, we could think of ways to switch them off using drugs. These drugs could be used as anti-viral treatments.

细胞可以被认为是岛屿——它们与外界隔绝，因为它们有一层不让任何东西通过的质膜。然而，细胞已经进化出微小的入口，让物质以一种严格控制的方式进入。这些通道一直在工作，但当细胞需要分裂时，它们就会关闭。在这个时候不要犯错误是很重要的。否则，细胞可能会死亡，或者开始以一种不寻常的方式生长，导致癌症等疾病。没有人知道这种关闭是如何发生的。我们想用一台机器来研究传送门它能告诉我们传送门的所有组成部分。如果我们比较正常细胞和正在分裂的细胞的门脉这将告诉我们分裂细胞中缺失了哪些部分。然后我们就能发现细胞在分裂开始时是如何带走这些碎片的。我们认为这项工作很重要。这些通道被病毒劫持：它们利用通道感染细胞。如果我们知道细胞是如何关闭通道的，我们就能想到用药物关闭通道的方法。这些药物可用作抗病毒治疗。

项目成果

Mitotic inhibition of clathrin-mediated endocytosis.

• DOI: 10.1007/s00018-012-1250-8
• 发表时间: 2013-09
• 期刊: CELLULAR AND MOLECULAR LIFE SCIENCES
• 影响因子: 8
• 作者: Fielding, Andrew B.;Royle, Stephen J.
• 通讯作者: Royle, Stephen J.

Isolation of clathrin-coated vesicles from tissue culture cells.

从组织培养细胞中分离网格蛋白包被的囊泡。

• DOI: 10.1101/pdb.top074435
• 发表时间: 2014
• 期刊: Cold Spring Harbor protocols
• 作者: Borner GH

Mitotic moonlighting functions for membrane trafficking proteins.

• DOI: 10.1111/j.1600-0854.2011.01184.x
• 发表时间: 2011-07
• 期刊: Traffic (Copenhagen, Denmark)
• 作者: Royle SJ

An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.

• DOI: 10.7554/elife.00829
• 发表时间: 2014-02-18
• 期刊: eLife
• 影响因子: 7.7
• 作者: Kaur S;Fielding AB;Gassner G;Carter NJ;Royle SJ
• 通讯作者: Royle SJ

An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis

• DOI: 10.1101/001701
• 发表时间: 2014-01
• 期刊: eLife
• 影响因子: 7.7
• 作者: S. Kaur;A. Fielding;Gisela Gassner;N. J. Carter;S. Royle