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REGULATION OF REAGINIC ANTIBODY RESPONSE

反应性抗体反应的调节

基本信息

批准号：
3124922
负责人：
KIMISHIGE ISHIZAKA
金额：
$ 24.93万
依托单位：
LA JOLLA INSTITUTE FOR IMMUNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-02-01 至 1992-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3124922
关键词：
antibody formation antibody receptor antigen presenting cell glycosylation helper T lymphocyte human subject hybridomas hypersensitivity immunoglobulin E immunoglobulin G immunoregulation immunosuppression laboratory mouse laboratory rat lymphokines suppressor T lymphocyte

项目摘要

The purpose of this research is to elucidate the mechanisms involved in the regulation of the IgE response, and to find some maneuvors to suppress the IgE antibody formation to allergens. During the current grant period, we found that a lipocortin-like lymphokine, i.e., glycosylation inhibiting factor (GIF), suppressed the primary IgE and IgG antibody responses in the mouse, and diminished the on-going IgE antibody formation. Analysis of cellular mechanisms for the immunosuppression indicated that GIF facilitated the generation of antigen-specific suppressor T cells which formed their own GIF. In the present proposal, attempts will be made to generate antigen-specific suppressor T cells in vitro from antigen-primed T cells, and to elucidate the cellular and molecular mechanisms involved. Experiments will be carried out to determine whether GIF may change the function of antigen-primed T cells, and may affect the antigen-presenting cells. It was also shown that antigen-specific suppressor T cells form GIF which has affinity for the antigen, and that the antigen- specific GIF suppresses the in vivo antibody response in a carrier- specific manner. Mouse T cell hybridomas were constructed from an ovalbumin-primed mouse T cell clone. One of the T cell hybridomas form antigen-specific GIF upon incubation with antigen-pulsed syngenic macrophages. In this proposal, antigen- specific GIF will be obtained from the T cell hybridomas, and physicochemical properties of the molecules will be characterized. Comparisons will be made between the antigen- specific GIF and antigen-specific T suppressor factor (TsF) described by other investigators. Analysis will be made to determine whether the MHC compatibility between the cell source of antigen-specific GIF and target cells is essential for the carrier-specific suppression. Based on the findings obtained in mouse lymphocyte systems, attempts will be made to generate antigen-specific suppressor T cells in vitro from the peripheral blood T cells of allergic patients, and to construct human antigen- specific T cell hybridomas which will produce allergen-specific GIF that may suppress the antibody response to the allergen.

本研究的目的是阐明其机制