REGULATION OF REAGINIC ANTIBODY RESPONSE

本研究的目的是阐明其作用机制。 参与调节免疫球蛋白E应答，并找到一些 抑制变应原IgE抗体形成的策略。 在目前的资助期内，我们发现一种脂可的样蛋白 淋巴因子，即糖基化抑制因子(GIF)被抑制 小鼠的初次免疫球蛋白E和免疫球蛋白抗体反应 减少正在进行的IgE抗体的形成。分析 免疫抑制的细胞机制表明 GIF促进抗原特异性抑制T细胞的产生 形成自己的GIF的细胞。在本提案中， 将尝试产生抗原特异性抑制物T 细胞从抗原诱导的T细胞中分离出来，并阐明 涉及细胞和分子机制。实验将是 执行以确定GIF是否会改变 抗原诱导的T细胞，并可能影响抗原提呈 细胞。研究还表明，抗原特异性抑制T细胞 形成与该抗原有亲和力的GIF，而该抗原- 特异性GIF抑制携带者体内抗体反应- 具体的方式。小鼠T细胞杂交瘤的构建 卵清蛋白免疫的小鼠T细胞克隆。T细胞中的一个 杂交瘤与抗原结合形成抗原特异性GIF 抗原致敏的同源巨噬细胞。在这项提议中，抗原- 将从T细胞杂交瘤中获得特异性GIF，以及 分子的物理化学性质将是 特色化的。我们将对抗原进行比较- 特异性GIF和抗原特异性T抑制因子(TSF) 其他调查人员描述了这一点。将对以下内容进行分析 确定MHC细胞之间是否兼容 抗原特异性GIF和靶细胞的来源对于 特定于运营商的抑制。基于在中获得的研究结果 小鼠的淋巴细胞系统，会尝试产生 外周血中抗原特异性抑制性T细胞的体外培养 过敏患者的血液T细胞，并构建人类抗原- 产生变应原特异性的特异性T细胞杂交瘤 可能抑制过敏原抗体反应的GIF。

The purpose of this research is to elucidate the mechanisms involved in the regulation of the IgE response, and to find some maneuvors to suppress the IgE antibody formation to allergens. During the current grant period, we found that a lipocortin-like lymphokine, i.e., glycosylation inhibiting factor (GIF), suppressed the primary IgE and IgG antibody responses in the mouse, and diminished the on-going IgE antibody formation. Analysis of cellular mechanisms for the immunosuppression indicated that GIF facilitated the generation of antigen-specific suppressor T cells which formed their own GIF. In the present proposal, attempts will be made to generate antigen-specific suppressor T cells in vitro from antigen-primed T cells, and to elucidate the cellular and molecular mechanisms involved. Experiments will be carried out to determine whether GIF may change the function of antigen-primed T cells, and may affect the antigen-presenting cells. It was also shown that antigen-specific suppressor T cells form GIF which has affinity for the antigen, and that the antigen- specific GIF suppresses the in vivo antibody response in a carrier- specific manner. Mouse T cell hybridomas were constructed from an ovalbumin-primed mouse T cell clone. One of the T cell hybridomas form antigen-specific GIF upon incubation with antigen-pulsed syngenic macrophages. In this proposal, antigen- specific GIF will be obtained from the T cell hybridomas, and physicochemical properties of the molecules will be characterized. Comparisons will be made between the antigen- specific GIF and antigen-specific T suppressor factor (TsF) described by other investigators. Analysis will be made to determine whether the MHC compatibility between the cell source of antigen-specific GIF and target cells is essential for the carrier-specific suppression. Based on the findings obtained in mouse lymphocyte systems, attempts will be made to generate antigen-specific suppressor T cells in vitro from the peripheral blood T cells of allergic patients, and to construct human antigen- specific T cell hybridomas which will produce allergen-specific GIF that may suppress the antibody response to the allergen.