REGULATION OF REAGINIC ANTIBODY RESPONSE

本研究的目的是阐明其机制 参与调节IgE反应，并找到一些 抑制过敏原IgE抗体形成的抑制剂。 在目前的资助期间，我们发现一种脂皮质素样的 淋巴因子，即，抑制糖基化抑制因子 小鼠中的原发性IgE和IgG抗体应答，以及 减少了持续的IgE抗体形成。 分析 免疫抑制的细胞机制表明， GIF促进抗原特异性抑制T细胞的产生， 这些细胞形成了自己的GIF。 在本提案中， 将尝试产生抗原特异性抑制因子T 细胞在体外从抗原致敏的T细胞，并阐明 涉及的细胞和分子机制。 实验将 进行，以确定GIF是否可以改变的功能， 抗原引发的T细胞，并可能影响抗原呈递 细胞 还表明抗原特异性抑制性T细胞 形成对抗原具有亲和力的GIF，并且抗原- 特异性GIF抑制载体中的体内抗体应答- 具体方式。 小鼠T细胞杂交瘤构建自 卵清蛋白引发的小鼠T细胞克隆。 其中一个T细胞 杂交瘤在与下列物质孵育后形成抗原特异性GIF 抗原脉冲的同系巨噬细胞。 在这个提议中，抗原- 特异性GIF将从T细胞杂交瘤中获得， 分子的物理化学性质将是 表征了 我们会比较抗原- 特异性GIF和抗原特异性T抑制因子（TsF） 其他研究人员描述。 将进行分析， 确定细胞之间的MHC相容性 抗原特异性GIF和靶细胞的来源是至关重要的， 载波特定抑制 根据在 小鼠淋巴细胞系统，将尝试产生 外周血抗原特异性抑制性T细胞 过敏患者的血液T细胞，并构建人抗原- 特异性T细胞杂交瘤将产生变应原特异性 GIF可以抑制对过敏原的抗体反应。

The purpose of this research is to elucidate the mechanisms involved in the regulation of the IgE response, and to find some maneuvors to suppress the IgE antibody formation to allergens. During the current grant period, we found that a lipocortin-like lymphokine, i.e., glycosylation inhibiting factor (GIF), suppressed the primary IgE and IgG antibody responses in the mouse, and diminished the on-going IgE antibody formation. Analysis of cellular mechanisms for the immunosuppression indicated that GIF facilitated the generation of antigen-specific suppressor T cells which formed their own GIF. In the present proposal, attempts will be made to generate antigen-specific suppressor T cells in vitro from antigen-primed T cells, and to elucidate the cellular and molecular mechanisms involved. Experiments will be carried out to determine whether GIF may change the function of antigen-primed T cells, and may affect the antigen-presenting cells. It was also shown that antigen-specific suppressor T cells form GIF which has affinity for the antigen, and that the antigen- specific GIF suppresses the in vivo antibody response in a carrier- specific manner. Mouse T cell hybridomas were constructed from an ovalbumin-primed mouse T cell clone. One of the T cell hybridomas form antigen-specific GIF upon incubation with antigen-pulsed syngenic macrophages. In this proposal, antigen- specific GIF will be obtained from the T cell hybridomas, and physicochemical properties of the molecules will be characterized. Comparisons will be made between the antigen- specific GIF and antigen-specific T suppressor factor (TsF) described by other investigators. Analysis will be made to determine whether the MHC compatibility between the cell source of antigen-specific GIF and target cells is essential for the carrier-specific suppression. Based on the findings obtained in mouse lymphocyte systems, attempts will be made to generate antigen-specific suppressor T cells in vitro from the peripheral blood T cells of allergic patients, and to construct human antigen- specific T cell hybridomas which will produce allergen-specific GIF that may suppress the antibody response to the allergen.