DEGRANULATION FUNCTIONS OF CYTOTOXIC T LYMPHOCYTES

细胞毒性 T 淋巴细胞的脱颗粒功能

• 批准号: 3125853
• 负责人: WILLIAM R CLARK
• 金额: $ 17.65万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-30 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3125853
• 关键词: cell mediated lymphocytolysis test; chromium; cytotoxic T lymphocyte; gene expression; genetic manipulation; granule; helper T lymphocyte; laboratory mouse; leukocyte activation /transformation; lymphokines; plant virus; protein biosynthesis; protein sequence; protein structure function; radionuclides; radiotracer; tissue /cell culture

Cytotoxic T lymphocytes (CTLs) that have been exposed for several days to high concentrations of IL-2 either in vitro or in vivo become highly granular in nature. Considerable attention has been focused on the possibility that these IL-2 induced granules might contain a cytotoxin that could explain how CTLs kill their target cells. How such a mechanism would fit into CTL killing in general is still controversial. What cannot be questioned, however, is that CTLs do not undergo a degranulation process during target cell killing. The contents of CTL granules have been only crudely analyzed. While it is certain that a number of different serine esterases reside in granules, little else is known. We recently began an extensive search for biologically interesting activities released during CTL degranulation. We have defined three activities functionally, and now propose detailed biological, biochemical and molecular genetic analyses of these activities. The three activities we have defined are: CTL inhibition, which is probably a feedback regulatory mechanism for controlling CTL function; T helper cell inhibitin, which may be involved in CD8+ T cell suppression of helper function; and viral inhibitin, which drastically inhibits the ability of mature viral particles to infect cells. Understanding the biochemical basis of these activities will greatly broaden our understanding of CTL function.

细胞毒性T淋巴细胞（ctl）已经暴露了几个

项目成果

The role of accessory molecules in T-helper activation induced by antigen, lectin, or CD3 antibodies.

辅助分子在抗原、凝集素或 CD3 抗体诱导的 T 辅助细胞激活中的作用。

• DOI: 10.1016/0008-8749(88)90187-6
• 发表时间: 1988
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Torbett,BE;Clark,WR
• 通讯作者: Clark,WR

Cloned cytotoxic T lymphocyte target cells fail to induce early activation events in effector cytotoxic T lymphocytes.

克隆的细胞毒性T淋巴细胞靶细胞无法诱导效应细胞毒性T淋巴细胞的早期激活事件。

• DOI: 10.1016/0008-8749(88)90265-1
• 发表时间: 1988
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Ostergaard,H;Gorman,K;Clark,WR
• 通讯作者: Clark,WR

The relationship between plasma membrane lipid composition and physical-chemical properties. III. Detailed physical and biochemical analysis of fatty acid-substituted EL4 plasma membranes.

质膜脂质组成与理化性质之间的关系。

• DOI: 10.1016/0005-2736(82)90255-3
• 发表时间: 1982
• 期刊: Biochimica et biophysica acta
• 作者: Poon,R;Clark,WH
• 通讯作者: Clark,WH

Evidence for multiple lytic pathways used by cytotoxic T lymphocytes.

细胞毒性 T 淋巴细胞使用多种裂解途径的证据。

• 发表时间: 1989
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Ostergaard,HL;Clark,WR
• 通讯作者: Clark,WR

Class I MHC antigens in the generation and expression of promiscuous cytotoxic cell function.

I 类 MHC 抗原在混杂细胞毒性细胞功能的产生和表达中的作用。

• 发表时间: 1986
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Kane,KP;Clark,WR
• 通讯作者: Clark,WR