EVALUATION OF MOSQUITO FLIGHT BEHAVIOR IN A WIND TUNNEL

苔藓虫的寄主寻找行为还不太清楚。 使用的线索 蚊子寻找宿主的飞行行为和寻找宿主的飞行行为 这些线索仍然模糊不清。 事实上，许多人类病原体携带 并通过蚊子传播使得了解它们的基本生物学 关键，特别是现在，经典的控制方法似乎是 在全球范围内失败。 这里提出的研究将试图揭示一些 蚊子寻找宿主的基本机制 和一般的飞行行为 结果将有助于改善我们的 了解疾病的传播和我们控制疾病的能力 蚊子 蚊子飞行行为的种间比较和评价， 提出了 的寄主寻找、扩散和吸血行为 将对几种重要的病媒和害虫蚊子进行评估， 大型（11 × 2.4 × 2.4米）室外风洞已建成， 测试. 这种独特的自动化隧道被设计为嗅觉仪， 在蚊子释放点的上风处有一对选择室。 单个、多个或竞争性刺激（即，视觉，听觉， 嗅觉）可以（同时）呈现，它们对 蚊子的定向和寻找宿主的飞行行为， 蚊子对每一种的反应比较。 我们计划比较 白天活动与黄昏/黄昏活动蚊子的行为， 同一物种的定居蚊子、F1蚊子和野生蚊子的飞行行为 以及标记的飞行行为（即，用荧光粉）和 没有标记的蚊子 我们还将研究主机的现象 偏好，并试图确定背后的行为基础， 在野外观察了几种蚊子的进食模式。 最后，通过这个风洞系统，我们将能够评估 实地观察蚊子在更可控的环境中的行为。 为 例如，我们将试图确定我们最近的原因， 观察到猪油罐陷阱与可访问的主机，在被困 蚊子可以吸血，捕获的蚊子数量可达10倍 作为陷阱与难以接近的主机，蚊子无法接触 或者进食

Mosquito host-seeking behavior is not well understood. The cues used by mosquitoes to locate hosts and the flight behaviors involved in finding those cues remain obscure. The fact that many human pathogens are carried and transmitted by mosquitoes makes understanding their basic biology essential, especially now that the classical control methods seem to be failing worldwide. The studies proposed here will attempt to uncover some of the basic underlying mechanisms associated with mosquito host-seeking and general flight behavior. Results will help to improve our understanding of disease transmission and our capacity to control mosquitoes. An interspecific comparison and evaluation of mosquito flight behavior is proposed. The host-seeking, dispersal, and blood-feeding behaviors of several important vector and pest mosquito species will be evaluated with a large (11 X 2.4 X 2.4 meters) outdoor wind tunnel which has been build and tested. This unique, automated tunnel is designed as an olfactometer with a pair of choice chambers upwind of the mosquito release point. Individual, multiple or competing stimuli (i.e., visual, auditory, olfactory) can be presented (simultaneously) and their importance to mosquito orientation and host-seeking flight behavior evaluated based on the comparative response of mosquitoes to each. We plan to compare the behaviors of day-active vs. crepuscular/nocturnally-active mosquitoes, the flight behavior of colonized, F1, and wild mosquitoes of the same species and the flight behavior of marked (i.e., with fluorescent powder) and unmarked mosquitoes. We also will investigate the phenomenon of host preference and attempt to determine the behavioral basis behind the observed feeding patterns of several mosquito species in the field. Finally, with this wind tunnel system we will be able to evaluate field-observed mosquito behaviors in a more controlled setting. For example, we will attempt to determine the reasons behind our recent observation that lard-can traps with accessible hosts, upon which trapped mosquitoes can blood-feed, capture up to 10 times the number of mosquitoes as traps with inaccessible hosts, on which mosquitoes cannot make contact or feed.