STUDY OF A HUMAN B LYMPHOCYTE SUBSET FOR IGG2

IGG2 人类 B 淋巴细胞亚群的研究

• 批准号: 3129040
• 负责人: Moon H. Nahm
• 金额: $ 8.65万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-04-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3129040
• 关键词: B lymphocyte; analytical method; autoimmune disorder; bacterial antigens; human tissue; hybridomas; immune complex diseases; immunodeficiency; immunoglobulin G; immunoglobulin genes; immunopathology diagnosis; leukemia; leukocyte activation /transformation; microorganism immunology; monoclonal antibody; plaque assay; radiation sensitivity; radioimmunoassay; tissue /cell culture

Many disease processes involve the complex immune system in various ways. The immune system is largely controlled by lymphocytes which are functionally diverse though morphologically uniform. This morphological uniformity hinders our effort in separating the complex immune system into simpler functional subunits which are easier to study. Hence clear identification of the functional subunits among lymphocytes should greatly aid our understanding immune functions. Following the evidence accumlated from animal studies described in this proposal, I will identify a human B lymphocyte subset producing IgG2 - an immunoglobulin class important for combating infection. To achieve this, my work is divided into 4 sections: A) DEVELOPMENT OF ANALYTICAL METHODS B) DEMONSTRATION OF FUNCTIONAL SUBSETS OF B LYMPHOCYTES C) ASSOCIATION OF THE FUNCTIONAL SUBSETS WITH KNOWN PHENOTYPIC MARKERS D) STUDY OF HYBRIDOMA ANTIBODIES RECOGNIZING THE FUNCTIONAL SUBSET. Clear demonstration of B lymphocyte subsets through this multipronged approach will help us understand the complex immune system and its relationship to medical problems. To a physician working in a diagnostic clinical laboratory, these new insights mean a possibility for new diagnostic and therapeutic approaches to many diseases including immunodeficiency diseases, infections, autoimmunity and malignancies such as leukemia. Indeed in a prior work, basic research into the immune response to group A carbohydrate antigen enabled us to improve the procedures for diagnosing group A streptococcal infection (J. Clin. Microbiol. 12:506, 1980).

许多疾病过程以各种方式涉及复杂的免疫系统。 免疫系统主要由淋巴细胞控制， 功能多样，但形态一致。 该形态 单一性阻碍了我们将复杂的免疫系统分离为 更简单的功能亚基，更容易研究。 因此， 在淋巴细胞中鉴定功能亚基， 帮助我们了解免疫功能。 根据证据， 从本提案中描述的动物研究中，我将确定一个人类B 产生IgG 2的淋巴细胞亚群-一种重要的免疫球蛋白类， 对抗感染。 为了实现这一目标，我的工作分为四个部分： A）分析方法的开发B）功能性证明 B淋巴细胞亚群C）功能亚群与 已知表型标志物D）杂交瘤抗体识别 函数子集。 明确显示B淋巴细胞亚群， 这种多管齐下的方法将帮助我们理解复杂免疫 系统及其与医疗问题的关系。 一位医生， 诊断临床实验室，这些新的见解意味着一种可能性， 许多疾病的新诊断和治疗方法， 免疫缺陷疾病、感染、自身免疫和恶性肿瘤， 白血病 事实上，在之前的工作中，对免疫系统的基础研究 对A组碳水化合物抗原的反应使我们能够改善 用于诊断A组链球菌感染的方法（J. Clin. Microbiol. 12：506，1980）。