NANOCELL

纳米细胞

• 批准号: BB/I004831/1
• 负责人: Richard Berry
• 金额: $ 40.75万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI004831%2F1
• 关键词: NANOCELL

The overall goal of the NANOCELL consortium is to engineer biological components that can be assembled into artificial membrane-bound vesicles that have some of the functions of living cells. These NANOCELLS will be prototype biochemical factories for future applications in bio-nanotechnology. The membrane itself is the most important component. Another important component is F1FO (ATP-synthase). F1FO can be thought of as two rotary motors connected in opposition. Normally in nature the ion-driven FO generates more torque, and forces the ATP-driven F1 in reverse, thereby synthesizing ATP, the 'energy currency' of the cell. One goal of this part of the consortium (IP4) is to test and characterize the function of membranes containing F1FO in NANOCELLS, using fluorescence methods including single-NANOCELL fluorescence microscopy. F1FO and other membrane protein components will also be tested and characterized at the single-molecule level in droplet-on-hydrogel bilayers, which allow large planar membranes and membrane proteins that they contain to be observed with high-resolution optical microscopy. The final goal of IP4 is to use one or both of the F1FO rotary motors to drive a propeller that will allow NANOCELLS to move under their own power. The lab of IP4 is one of the world leaders in the study of the bacterial flagellar motor, which performs the same role in flagellated bacteria, and will use this expertise to guide the development of a swimming module for NANOCELLS.

NANOCELL 联盟的总体目标是设计可组装成具有活细胞某些功能的人工膜结合囊泡的生物成分。这些纳米细胞将成为未来生物纳米技术应用的原型生化工厂。膜本身是最重要的组成部分。另一个重要成分是 F1FO（ATP 合酶）。 F1FO 可以被认为是两个反向连接的旋转电机。通常在自然界中，离子驱动的 FO 会产生更大的扭矩，并迫使 ATP 驱动的 F1 反向，从而合成 ATP，即细胞的“能量货币”。该联盟 (IP4) 的这一部分的目标之一是使用包括单 NANOCELL 荧光显微镜在内的荧光方法来测试和表征 NANOCELL 中含有 F1FO 的膜的功能。 F1FO 和其他膜蛋白成分也将在水凝胶双层液滴的单分子水平上进行测试和表征，这使得可以用高分辨率光学显微镜观察大平面膜及其所含的膜蛋白。 IP4 的最终目标是使用一个或两个 F1FO 旋转电机来驱动螺旋桨，使 NANOCELLS 在自身动力的作用下移动。 IP4 实验室是细菌鞭毛马达研究领域的世界领先者之一，该实验室在鞭毛细菌中发挥着相同的作用，并将利用这一专业知识来指导纳米细胞游泳模块的开发。

项目成果

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

• DOI: 10.3791/56909
• 发表时间: 2018-04-05
• 期刊: Journal of visualized experiments : JoVE
• 作者: Galkin MA;Russell AN;Vik SB;Berry RM;Ishmukhametov RR
• 通讯作者: Ishmukhametov RR

Comparison between single-molecule and X-ray crystallography data on yeast F1-ATPase.

• DOI: 10.1038/srep08773
• 发表时间: 2015-03-10
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Steel BC;Nord AL;Wang Y;Pagadala V;Mueller DM;Berry RM
• 通讯作者: Berry RM

A Simple low-cost device enables four epi-illumination techniques on standard light microscopes.

一种简单的低成本设备可在标准光显微镜上采用四种表弹技术。

• DOI: 10.1038/srep20729
• 发表时间: 2016-02-08
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Ishmukhametov RR;Russell AN;Wheeler RJ;Nord AL;Berry RM
• 通讯作者: Berry RM

A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes.

• DOI: 10.1038/ncomms13025
• 发表时间: 2016-10-06
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Ishmukhametov, Robert R.;Russell, Aidan N.;Berry, Richard M.
• 通讯作者: Berry, Richard M.