DIAGNOSIS OF NEUROCYSTICERCOSIS

要求提供三年的支助，以开展研究， 囊虫病的诊断方法，特别强调 脑囊虫病的诊断 初步研究表明， 使用酶联免疫吸附测定（ELISA）用于 检测感染人血清中的抗体 猪带绦虫 该测定是对现有血清诊断方法的改进。 通过优化试剂的程序。 的一个主要目标 研究目的是鉴定T.可以提高 血清学诊断程序的准确性。 第二个主要目标 本研究旨在评价用于检测和定量 人脑脊液（CSF）中幼虫抗原 脑囊虫病 为了完成这方面的研究， 每年将确定大约20名神经囊尾蚴病患者， 1994年在安蒂奥基亚大学医院接受治疗。 哥伦比亚，麦德林。 将在术前、术中和术后采集血清和CSF。 治疗后，并在CAT扫描的帮助下， 幼虫数量/体积和使用ELISA测定的治疗有效性 程序. 希望能研制出一种简单、廉价的ELISA试剂盒， 用于诊断脑囊虫病。

Three years of support are requested for studies to develop improved methods for the diagnosis of cysticercosis with special emphasis on the diagnosis of neurocysticercosis. Preliminary studies demonstrate the utility of using an enzymed-linked immunosorbant assay (ELISA) for detection of antibodies in the serum of people infected with the larvae of Taenia solium. This assay is an improvement over existing sero-diagnostic procedures through optimization of the reagents. A major goal of the research is to identify antigens of larval T. solium which can improve the accuracy of serodiagnostic procedurees. The second principal goal of the study is to evaluate an ELISA procedure for detection and quantification of antigens of larvae in the cerebrospinal fluid (CSF) of persons with neurocysticercosis. To accomplish this aspect of the research, approximately 20 neurocysticercotic patients per year will be identified and treated in the University Hospital of the Universidad de Antioquia in Medellin, Colombia. Sera and CSF will be collected prior to, during, and following therapy, and with the aid of CAT scans, possible correlations of number/volume of larvae and effectiveness of therapy determined using ELISA procedures. It is hoped that a simple, inexpensive ELISA kit can be developed for the diagnosis of neurocysticercosis.