DIAGNOSIS OF NEUROCYSTICERCOSIS

神经囊虫病的诊断

• 批准号: 3133003
• 负责人: Raymond E. Kuhn
• 金额: $ 12.64万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3133003
• 关键词: cerebrospinal fluid; computed axial tomography; enzyme linked immunosorbent assay; helminthic antigen; high performance liquid chromatography; human subject; human therapy evaluation; immunologic assay /test; larva; nervous system infection; parasitic disease diagnosis; serology /serodiagnosis

Three years of support are requested for studies to develop improved methods for the diagnosis of cysticercosis with special emphasis on the diagnosis of neurocysticercosis. Preliminary studies demonstrate the utility of using an enzymed-linked immunosorbant assay (ELISA) for detection of antibodies in the serum of people infected with the larvae of Taenia solium. This assay is an improvement over existing sero-diagnostic procedures through optimization of the reagents. A major goal of the research is to identify antigens of larval T. solium which can improve the accuracy of serodiagnostic procedurees. The second principal goal of the study is to evaluate an ELISA procedure for detection and quantification of antigens of larvae in the cerebrospinal fluid (CSF) of persons with neurocysticercosis. To accomplish this aspect of the research, approximately 20 neurocysticercotic patients per year will be identified and treated in the University Hospital of the Universidad de Antioquia in Medellin, Colombia. Sera and CSF will be collected prior to, during, and following therapy, and with the aid of CAT scans, possible correlations of number/volume of larvae and effectiveness of therapy determined using ELISA procedures. It is hoped that a simple, inexpensive ELISA kit can be developed for the diagnosis of neurocysticercosis.

需要三年的支持，以开发改进的研究 囊虫病诊断方法的探讨 脑囊虫病的诊断。初步研究显示， 酶联免疫吸附试验(ELISA法)用于检测 日本血吸虫幼虫感染者血清抗体的检测 猪带绦虫。这项检测是对现有的血清诊断方法的改进。 通过对试剂的优化，建立了一系列的检测方法。世界银行的一个主要目标 研究目的是鉴定幼虫的抗原，以提高幼虫的抗原性。 血清诊断程序的准确性。的第二个主要目标 本研究旨在评价一种检测和定量检测甲胎蛋白的ELISA方法。 脑炎患者脑脊液中幼虫抗原的检测 脑囊虫病。为了完成这方面的研究， 每年将确定大约20名脑囊虫病患者。 并在安提奥基亚大学的大学医院接受治疗 哥伦比亚的麦德林。血清和脑脊液将在治疗前、治疗期间和治疗前采集。 在治疗后，借助CAT扫描，可能的相关性 用酶联免疫吸附试验测定幼虫数量/体积和治疗效果 程序。人们希望一种简单、廉价的酶联免疫吸附试验试剂盒能够 为诊断脑囊虫病而开发的。