IMMUNOBIOLOGY OF CHAGAS' DISEASE

南美锥虫病的免疫生物学

• 批准号: 3480723
• 负责人: Raymond E. Kuhn
• 金额: $ 21.86万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3480723
• 关键词: T lymphocyte; Trypanosoma cruzi; antigen antibody reaction; cell mediated cytotoxicity; cellular immunity; cellular pathology; clone cells; complement; enzyme linked immunosorbent assay; gel electrophoresis; genetic strain; helper T lymphocyte; hybridomas; immunogenetics; immunoregulation; immunosuppression; interleukin 2; laboratory mouse; laboratory rabbit; protozoal antigen; radioimmunoassay; tissue /cell culture; trypanosomiasis

Experiments are proposed to examine several aspects of the immunobiology of the host-parasite relationship in experimental Chagas' disease (American trypanosomiasis). In a continuation of previous studies, the cellular basis of immunosuppression in mammals infected with Trypanosoma cruzi will be examined with special emphasis on T helper cells and their effector functions as related to their production and sensitivity to interleukin 2. The role of T cells with phenotypes indicative of suppressor and cytotoxic T cells will be examined in an attempt to determine the role of these sub-populations of lymphocytes. Studies with T helper cells (L3T4) and Lyt 2,3+ T cells and be performed using long-term T cell lines and/or clones in vitro and in vivo. There effects will be assessed by their ability to affect longevity and parasitemia in highly susceptible and resistant strains of mice. Experiments and also planned to re-examine the role and significance of antibody-dependent, complement-mediated effector functions in lysis of trypomastigotes of T. cruzi as recent results suggest that the significance of antibody in immunity to T. cruzi is highly dependent on the strain of parasite used in such studies.

建议进行实验，以检查以下几个方面 实验性宿主-寄生虫关系的免疫生物学研究 查加斯病(美洲锥虫病)。在...的延续中 以前的研究表明，免疫抑制的细胞学基础 感染克氏锥虫的哺乳动物将接受检查 特别强调T辅助细胞及其效应器的功能 与它们的产生和对白介素2的敏感性有关。 表型为抑制性T细胞的T细胞的作用 将对细胞毒性T细胞进行检查，试图确定 这些淋巴细胞亚群的作用。关于T的研究 辅助细胞(L3T4)和Lyt 2，3+T细胞，并使用 体外和体内长期T细胞系和/或克隆。那里 效果将通过它们影响寿命和 高度敏感和耐药品系小鼠的寄生虫血症。 实验，还计划重新检查角色和 抗体依赖、补体介导的意义 效应器在克氏锥虫裂解锥虫中的作用 结果提示，抗体在T。 克鲁兹病毒高度依赖于在这种情况下使用的寄生虫菌株。 学习。