IMMUNOBIOLOGY OF CHAGAS' DISEASE

南美锥虫病的免疫生物学

• 批准号: 3480720
• 负责人: Raymond E. Kuhn
• 金额: $ 21.06万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-08-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3480720
• 关键词: T lymphocyte; Trypanosoma cruzi; antigen antibody reaction; cell mediated cytotoxicity; cellular immunity; cellular pathology; clone cells; complement; enzyme linked immunosorbent assay; gel electrophoresis; genetic strain; helper T lymphocyte; hybridomas; immunogenetics; immunoregulation; immunosuppression; interleukin 2; protozoal antigen; radioimmunoassay; tissue /cell culture; trypanosomiasis

Experiments are proposed to examine several aspects of the immunobiology of the host-parasite relationship in experimental Chagas' disease (American trypanosomiasis). In a continuation of previous studies, the cellular basis of immunosuppression in mammals infected with Trypanosoma cruzi will be examined with special emphasis on T helper cells and their effector functions as related to their production and sensitivity to interleukin 2. The role of T cells with phenotypes indicative of suppressor and cytotoxic T cells will be examined in an attempt to determine the role of these sub-populations of lymphocytes. Studies with T helper cells (L3T4) and Lyt 2,3+ T cells and be performed using long-term T cell lines and/or clones in vitro and in vivo. There effects will be assessed by their ability to affect longevity and parasitemia in highly susceptible and resistant strains of mice. Experiments and also planned to re-examine the role and significance of antibody-dependent, complement-mediated effector functions in lysis of trypomastigotes of T. cruzi as recent results suggest that the significance of antibody in immunity to T. cruzi is highly dependent on the strain of parasite used in such studies.

提出实验来检查的几个方面， 实验动物宿主-寄生虫关系的免疫生物学 恰加斯病（美洲锥虫病）。 在延续 以前的研究，免疫抑制的细胞基础， 感染克氏锥虫的哺乳动物将被检查， 特别强调T辅助细胞及其效应器功能， 与它们的产生和对白细胞介素2的敏感性有关。 的 表型指示抑制因子的T细胞和 将检查细胞毒性T细胞以试图确定 这些淋巴细胞亚群的作用。 研究T 辅助细胞（L3 T4）和Lyt 2，3 + T细胞，并使用 体外和体内长期T细胞系和/或克隆。 那里 影响将通过其影响寿命的能力进行评估， 高敏感和耐药品系小鼠的寄生虫血症。 实验并还计划重新审视该角色， 抗体依赖性、补体介导 在T. cruzi最近 结果提示，T. cruzi高度依赖于在这种情况下使用的寄生虫菌株， 问题研究