IMMUNOCHEMICAL MEASUREMENT OF AIRBORNE ALLERGENS

空气中过敏原的免疫化学测量

• 批准号: 3131197
• 负责人: CHARLES E. REED
• 金额: $ 9.6万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131197
• 关键词: Ambrosia; Ascomycetes; Basidiomycetes; Gramineae; air filtration; air sampling /monitoring; airborne allergen; antigens; climate; dust; human tissue; immunochemistry; immunoglobulin E; plants; pollen; radioimmunoassay; seasons

Up to now, quantitation of airborne antigen has relied on counting pollens and mold spores identifiable morphologically under the microscope or by their characteristics in culture. Availability of quantitative immunochemical sampling techniques now allows measurement of any particulate antigen providing reference standards exist and serum pools contain IgE antibody can be assembled. Our method uses an Accu-Vol sampler to collect airborne dust particles 0.3 microns and larger or a similar apparatus designed for quiet indoor use. Antigens are eluted from the fiberglass filter sheet and assayed by RAST inhibition. In Rochester, MN, we will measure Alternaria (and Alt-1) daily from April 1st to November 1st, 1984 or 1985 and determine the size of the allergen containing particles on three days each month using an Anderson sampling head. We will also assay for rye grass group 1, birch and oak pollens. The samples will be assayed also for airborne amorphous insect antigens. Indoors we will assay five homes weekly throughout the year for cat and house dust mite antigens. In collaboration with investigators in New Orleans and Tucson, we will assay ragweed, Alternaria, rye grass 1 and Bermuda grass and compare the seasonal levels at different sites. In collaboration with investigators in New York and San Francisco, we will measure levels of mite and cockroach antigens indoors and determine climatic and seasonal variations in indoor or concentrations of these antigens.

到目前为止，空气中抗原的定量一直依赖于对花粉的计数 和霉菌孢子在显微镜下或通过 在文化上的特点。 提供定量 免疫化学取样技术现在允许测量任何 存在提供参考标准品的颗粒抗原和血清池 含有IgE的抗体可以被组装。 我们的方法使用一个Accu-Rolling采样器 收集0.3微米或更大的空气尘埃颗粒或类似的 为安静的室内使用而设计的装置。抗原从免疫球蛋白中洗脱。 玻璃纤维滤片，并通过RAST抑制进行测定。 在明尼苏达州的罗切斯特， 我们将从4月1日至11月每天测量链格孢菌（和Alt-1） 1984年或1985年，并确定含有过敏原的大小 使用安德森采样头，每月三天对颗粒进行采样。 我们 还将分析黑麦草组1、桦树和橡树花粉。 样品 还将测定空气传播的无定形昆虫抗原。 室内我们 一年中每周将对五户人家的猫尘和屋尘进行检测， 螨抗原 与新奥尔良的调查人员合作， 图森，我们将分析豚草，链格孢，黑麦草1和百慕大草 并比较不同地点的季节性水平。 协同 在纽约和旧金山弗朗西斯科的调查人员， 和蟑螂抗原，并确定气候和季节 室内或这些抗原的浓度的变化。