IMMUNOCHEMICAL ASSAY OF AEROALLERGENS

纵向研究表明，持续暴露于 患者患职业性哮喘后的过敏原可能 导致持续的呼吸道阻塞和高反应性 在暴露停止后的几个月或几年内。有充分的理由 想到持续暴露在家里或家里的过敏原 户外活动可能会产生类似的、不利的长期后果。 空气定量采样、颗粒大小测定和 无定形过敏原的免疫化学分析现在已经存在。在……里面 在之前的资助期，我们已经改进了空气采样策略 和设备，以及分析方法。我们已经测量了 工作场所的空气变应原在几次事故中 室外空气中的职业性过敏(链格孢霉和蛾子) 以及住宅的室内空气中。户外活动的意外结果 空气过敏原是豚草和橡树花粉的过敏原 在授粉期以外大量发生，以及 即使在开花期间，多达一半的过敏原是 与比花粉粒更小的颗粒有关。我们发现 植物其他部位的过敏原，如花、叶和茎。 此外，我们发现很容易测量到数量的蛾过敏原在 夏天和秋天。在室内，我们不仅发现了屋内的尘螨 和猫，但在哈莱姆的公寓里，有大量的 蟑螂和小鼠尿蛋白。一个新建的，紧凑的， 节能住宅的成本要高得多。 空气中的过敏原浓度比旧的、传统的房子要高。 在这家哈莱姆区的高效过滤器研究中 在公寓和老鼠区，似乎过敏原 在决定产量时，产量比过滤更重要 空气中的过敏原水平。 我们现在提出的研究有5个目标：1)确定 通风与透气的关系及与通风的关系 室内粉尘和猫变应原在空气中的浓度 5个城市约1,000套住宅；2)确定 家政活动对精矿浓度和矿物质含量的影响 家中粉尘和猫过敏原的颗粒大小；3)至 确定室外昆虫空气过敏原是否会在 波特兰昆虫IgE抗体阳性患者，或；4)至 确定昆虫是否授粉植物，蒲公英和 紫花苜蓿，从其他植物部位向空气中排放过敏原，如 豚草和橡木做；以及5)继续提炼和简化 空气采样和化验程序，以便它们可以成为普遍的 适用于执业过敏症专科医生。

Longitudinal studies have shown that continued exposure to the allergen after a patient has developed occupational asthma can lead to airway obstruction and hyperresponsiveness that persists for months or years after exposure ceases. There is good reason to think that continued exposure to allergens in the home or outdoors might have similar, adverse long term consequences. Techniques for quantitative air sampling, particle sizing, and immunochemical assay of amorphous allergens now exist. In previous grant periods we have refined air sampling strategies and equipment, as well as assay methods. We have measured aeroallergens in the work place in several occurrences of occupational allergy, in the outdoor air (Alternaria and moth) and in indoor air in dwellings. Unexpected results for outdoor aeroallergens were that both ragweed and oak pollen allergens occur in substantial amounts outside the pollenation period, and that even during flowering, up to half of the allergen is associated with particles smaller than pollen grains. We find allergen in other plant parts such as flowers, leaves and stems. Also, we find easily measurable quantities of moth allergen in the summer and fall. Indoors, we find not only house dust mite and cat but in Harlem tenements, substantial amounts of cockroach and mouse urinary protein. A newly built, tight, energy-efficient house had very much higher cat and mite allergen concentration in the air than older, conventional houses. In studies of high efficiency filters in this home, in Harlem tenements and in rat animal quarters, it appears that allergen production rate is more important than filtration in determining the allergen level in the air. We now propose studies with 5 aims: 1) to determine the relationship between ventilation and air permeation and the concentration of house dust mite and cat allergen in the air of approximately 1,000 dwelling in 5 cities; 2) to determine the effects of housekeeping activities on the concentration and particle size of mite and cat allergen in the home; 3) to determine whether outdoor insect aeroallergens cause asthma in patients with IgE antibody to insects in Portland, OR; 4) to determine whether the insect pollinated plants, dandelion and alfalfa, shed allergen into the air from other plants parts, as ragweed and oak do; and 5) to continue to refine and simplify the air sampling and assay procedures so they can become generally available to the practicing allergist.