IMMUNOCHEMICAL ASSAY OF AEROALLERGENS

空气过敏原的免疫化学测定

• 批准号: 3131201
• 负责人: CHARLES E. REED
• 金额: $ 12.3万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131201
• 关键词: Ambrosia; Diptera; Gramineae; air filtration; air sampling /monitoring; airborne allergen; antigens; asthma; cats; climate; cockroach; controlled environment; disease /disorder prevention /control; dust; electrofocusing; enzyme linked immunosorbent assay; human subject; immunoglobulin E; immunologic techniques; monoclonal antibody; paper chromatography; plants; pollen; pollution related respiratory disorder; pyroglyphid; radioimmunoassay; seasons

Longitudinal studies have shown that continued exposure to the allergen after a patient has developed occupational asthma can lead to airway obstruction and hyperresponsiveness that persists for months or years after exposure ceases. There is good reason to think that continued exposure to allergens in the home or outdoors might have similar, adverse long term consequences. Techniques for quantitative air sampling, particle sizing, and immunochemical assay of amorphous allergens now exist. In previous grant periods we have refined air sampling strategies and equipment, as well as assay methods. We have measured aeroallergens in the work place in several occurrences of occupational allergy, in the outdoor air (Alternaria and moth) and in indoor air in dwellings. Unexpected results for outdoor aeroallergens were that both ragweed and oak pollen allergens occur in substantial amounts outside the pollenation period, and that even during flowering, up to half of the allergen is associated with particles smaller than pollen grains. We find allergen in other plant parts such as flowers, leaves and stems. Also, we find easily measurable quantities of moth allergen in the summer and fall. Indoors, we find not only house dust mite and cat but in Harlem tenements, substantial amounts of cockroach and mouse urinary protein. A newly built, tight, energy-efficient house had very much higher cat and mite allergen concentration in the air than older, conventional houses. In studies of high efficiency filters in this home, in Harlem tenements and in rat animal quarters, it appears that allergen production rate is more important than filtration in determining the allergen level in the air. We now propose studies with 5 aims: 1) to determine the relationship between ventilation and air permeation and the concentration of house dust mite and cat allergen in the air of approximately 1,000 dwelling in 5 cities; 2) to determine the effects of housekeeping activities on the concentration and particle size of mite and cat allergen in the home; 3) to determine whether outdoor insect aeroallergens cause asthma in patients with IgE antibody to insects in Portland, OR; 4) to determine whether the insect pollinated plants, dandelion and alfalfa, shed allergen into the air from other plants parts, as ragweed and oak do; and 5) to continue to refine and simplify the air sampling and assay procedures so they can become generally available to the practicing allergist.

纵向研究表明，持续暴露于 患上职业性哮喘后， 导致持续气道阻塞和高反应性 几个月或几年后停止接触。 有充分理由 认为在家中持续接触过敏原， 户外活动可能会产生类似的长期不良后果。 定量空气取样、颗粒大小测定和 现在存在无定形过敏原免疫化学测定。 在 在过去的资助期内，我们改进了空气采样策略， 和设备，以及测定方法。 我们测量了 在工作场所的空气过敏原在几次发生 职业性过敏，在室外空气中（链格孢属和蛾） 和住宅的室内空气中。 户外活动的意外结果 空气变应原是豚草和橡树花粉变应原 在授粉期外大量发生， 即使在开花期间，多达一半的过敏原 与比花粉粒小的颗粒有关。 我们发现 过敏原在其他植物部分，如花，叶和茎。 此外，我们发现容易测量数量的蛾过敏原， 夏天和秋天。 在室内，我们不仅发现屋尘螨 但在哈莱姆的公寓里， 蟑螂和小鼠尿蛋白。 一个新建的，紧凑的， 节能型房屋的猫和螨的数量要高得多， 空气中的过敏原浓度比老式的传统房屋要低。 在这个哈莱姆的家中， 在公寓和大鼠动物宿舍，似乎过敏原， 在决定产量方面，生产率比过滤更重要。 空气中的过敏原水平 我们现在提出的研究有5个目标：1）确定 通风与空气渗透的关系 空气中屋尘螨和猫过敏原的浓度 5个城市约1，000人居住; 2）确定 内务活动对浓度和 家中螨和猫过敏原的颗粒大小; 3） 确定户外昆虫空气过敏原是否会导致哮喘， 波特兰，OR的昆虫IgE抗体患者; 4） 确定昆虫是否为植物授粉， 苜蓿，从其他植物部分向空气中释放过敏原， 豚草和橡树做;和5）继续完善和简化 空气采样和化验程序，使他们能够成为一般 提供给执业过敏症专科医生。