IMMUNOCHEMICAL MEASUREMENT OF AIRBORNE ALLERGENS

空气中过敏原的免疫化学测量

• 批准号: 3131198
• 负责人: CHARLES E. REED
• 金额: $ 7.82万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131198
• 关键词: Ambrosia; Ascomycetes; Basidiomycetes; Gramineae; air filtration; air sampling /monitoring; airborne allergen; antigens; climate; dust; human tissue; immunochemistry; immunoglobulin E; plants; pollen; radioimmunoassay; seasons

Up to now, quantitation of airborne antigen has relied on counting pollens and mold spores identifiable morphologically under the microscope or by their characteristics in culture. Availability of quantitative immunochemical sampling techniques now allows measurement of any particulate antigen providing reference standards exist and serum pools contain IgE antibody can be assembled. Our method uses an Accu-Vol sampler to collect airborne dust particles 0.3 microns and larger or a similar apparatus designed for quiet indoor use. Antigens are eluted from the fiberglass filter sheet and assayed by RAST inhibition. In Rochester, MN, we will measure Alternaria (and Alt-1) daily from April 1st to November 1st, 1984 or 1985 and determine the size of the allergen containing particles on three days each month using an Anderson sampling head. We will also assay for rye grass group 1, birch and oak pollens. The samples will be assayed also for airborne amorphous insect antigens. Indoors we will assay five homes weekly throughout the year for cat and house dust mite antigens. In collaboration with investigators in New Orleans and Tucson, we will assay ragweed, Alternaria, rye grass 1 and Bermuda grass and compare the seasonal levels at different sites. In collaboration with investigators in New York and San Francisco, we will measure levels of mite and cockroach antigens indoors and determine climatic and seasonal variations in indoor or concentrations of these antigens.

到目前为止，气载抗原的定量一直依赖于花粉计数。 和霉菌孢子，在显微镜下或通过 他们在文化上的特点。定量数据的可用性 免疫化学采样技术现在允许测量任何 有提供参考标准的颗粒抗原和血清池 含有IgE抗体的抗体可以组装。我们的方法使用Accu-Vol采样器 收集0.3微米或更大或类似的空气中的粉尘颗粒 专为安静的室内使用而设计的设备。抗原从细胞中洗脱出来 玻璃纤维滤片和Rast抑制法检测。在明尼苏达州罗切斯特市， 我们将从4月1日到11月每天测量Alternaria(和Alt-1) 1、1984或1985年，并确定含有以下物质的过敏原的大小 每月三天使用安德森采样头对颗粒进行采样。我们 还将化验黑麦草组1、桦树和橡树花粉。样品 还将检测空气传播的无定形昆虫抗原。在室内我们 将在一年中每周检测五个家庭的猫和房屋灰尘 螨类抗原。与新奥尔良和 图森，我们将化验豚草、交链孢菌、黑麦草1号和百慕大草 并比较不同地点的季节性水平。与 纽约和旧金山的调查人员，我们将测量螨类的水平 和蟑螂抗原在室内并决定气候和季节 这些抗原在室内或浓度上的变化。