PROTEIN DEGRADATION IN P. FALCIPARUM

我们研究的目标是阐明疟疾的传播途径， 寄生虫从宿主细胞的降解中获得游离氨基酸， 蛋白质，尤其是血红蛋白。 我们提出的研究分为两个主要方面 区域： 1.寄生虫蛋白酶的分离和性质研究 在寄生虫的食物链中血红蛋白的降解。 将表征来自恶性疟原虫的酸性和非酸性蛋白酶， 使用多种动力学/抑制方法。 的程度 这些蛋白酶单独地或共同地将血红蛋白降解成游离的 将测定氨基酸。 2.评价需要泛素的非溶酶体蛋白的作用 恶性疟原虫的周转率。 P.将检查恶性疟原虫是否存在ATP依赖性， 蛋白质降解的泛素需要途径。 中的作用 将确定血红蛋白降解的途径。

The goal of our research is to elucidate the pathways by which malarial parasites obtain free amino acids from degradation of the host cell's protein, especially hemoglobin. Our proposed studies fall into two major areas: 1. Isolation and Characterization of Parasite Proteases which Participate in the Degradation of Hemoglobin within the Parasite's Food Vacuole. Both acid and non-acid proteases from P. falciparum will be characterized, using a variety of kinetic/inhibition approaches. The extent to which these proteases, singly and collectively, degrade hemoglobin into free amino acids will be determined. 2. Evaluation of the Role of Ubiquitin-requiring, Nonlysosomal Protein Turnover in P. falciparum. P. falciparum will be examined for the presence of an ATP-dependent, ubiquitin-requiring pathway for protein degradation. The role of this pathway in the degradation of hemoglobin will be determined.