PROTEIN DEGRADATION IN P. FALCIPARUM

我们研究的目标是阐明疟疾 寄生虫从宿主细胞的降解中获得游离氨基酸 蛋白质，尤指血红蛋白。我们建议的研究分为两大类 地区： 1.参与的寄生虫蛋白酶的分离和性质 在寄生虫的食物液泡中降解血红蛋白。 恶性疟原虫的酸性和非酸性蛋白酶都将被鉴定， 使用各种动力学/抑制方法。在多大程度上 这些酶单独或共同地将血红蛋白降解成游离的。 氨基酸将被测定。 2.评估泛素需要的非溶酶体蛋白的作用 恶性疟原虫的周转。 恶性疟原虫将被检查是否存在依赖于ATP的， 蛋白质降解的泛素需要途径。这一点的作用 将确定血红蛋白降解的途径。

The goal of our research is to elucidate the pathways by which malarial parasites obtain free amino acids from degradation of the host cell's protein, especially hemoglobin. Our proposed studies fall into two major areas: 1. Isolation and Characterization of Parasite Proteases which Participate in the Degradation of Hemoglobin within the Parasite's Food Vacuole. Both acid and non-acid proteases from P. falciparum will be characterized, using a variety of kinetic/inhibition approaches. The extent to which these proteases, singly and collectively, degrade hemoglobin into free amino acids will be determined. 2. Evaluation of the Role of Ubiquitin-requiring, Nonlysosomal Protein Turnover in P. falciparum. P. falciparum will be examined for the presence of an ATP-dependent, ubiquitin-requiring pathway for protein degradation. The role of this pathway in the degradation of hemoglobin will be determined.