PROTEIN DEGRADATION IN P. FALCIPARUM

恶性疟原虫中的蛋白质降解

• 批准号: 3131137
• 负责人: DAVID L VANDER JAGT
• 金额: $ 9.02万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3131137
• 关键词: Plasmodium falciparum; aminoacid; enzyme structure; hemoglobin; host organism interaction; peptidases

The goal of our research is to elucidate the pathways by which malarial parasites obtain free amino acids from degradation of the host cell's protein, especially hemoglobin. Our proposed studies fall into two major areas: 1. Isolation and Characterization of Parasite Proteases which Participate in the Degradation of Hemoglobin within the Parasite's Food Vacuole. Both acid and non-acid proteases from P. falciparum will be characterized, using a variety of kinetic/inhibition approaches. The extent to which these proteases, singly and collectively, degrade hemoglobin into free amino acids will be determined. 2. Evaluation of the Role of Ubiquitin-requiring, Nonlysosomal Protein Turnover in P. falciparum. P. falciparum will be examined for the presence of an ATP-dependent, ubiquitin-requiring pathway for protein degradation. The role of this pathway in the degradation of hemoglobin will be determined.

我们研究的目的是阐明疟疾的致病途径