TN3 TRANSPOSITION IMMUNITY: A NEW GENETIC PHENOMENON
TN3 TRANSPOSITION IMMUNITY: A NEW GENETIC PHENOMENON
批准号:
3130856
负责人:
FRED L HEFFRON
金额:
$12.33万
依托单位国家:
美国
项目类别:
财政年份:
1984
资助国家:
美国
项目状态:
已结题
起止时间:
1984-03-01 至 1987-02-28
中文摘要
移位免疫是一种相对未被研究的遗传现象。
细菌。Tn3转位免疫是一种顺式作用机制,它限制
将Tn3插入到质粒中。我们已经证明了Tn3‘S末端重复序列可以
赋予对质粒的免疫力。然而,我们也发现,移位
免疫表型会受到寄主序列的影响。在此授予过程中
期间,我们将采用遗传方法,使用体外和
体内诱变,剖析免疫序列以及宿主和
也会影响免疫力的质粒序列。我们将确定是否
与Tn3相关的其他转座子表现出免疫力,如果是这样的话,确定
他们对彼此表现出交叉豁免权。我们将确定一个多大的
DNA分子Tn3是否能赋予免疫力以及是否还有其他参数
这也会影响豁免权。例如,一些自然产生的质粒
对Tn3免疫,但不会产生青霉素耐药性。穿过
这种遗传方法,以及额外的实验,我们将
区分转位免疫如何发挥作用的各种模型。
英文摘要
Transposition immunity is a relatively unstudied genetic phenomenon in
bacteria. Tn3 transposition immunity is a cis acting mechanism which limits
Tn3 insertion into a plasmid. We have shown that Tn3's terminal repeat can
confer immunity to a plasmid. However, we also find that the transposition
immunity phenotype can be affected by host sequences. During this granting
period we will pursue a genetic approach, using techniques of in vitro and
in vivo mutagenesis, to dissect the immunity sequence as well as host and
plasmid sequences which also affect immunity. We will determine whether
other transposons related to Tn3 show immunity and if so determine whether
they show cross immunity to each other. We will determine to how large a
DNA molecule Tn3 can confer immunity and whether there are other parameters
which also affect immunity. For example, some naturally occurring plasmids
are immune to Tn3 though they do not confer penicillin resistance. Through
this genetic approach, as well as additional experiments, we will
distinguish between the various models for how transposition immunity works.
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海外基金