IMMUNOBIOLOGY OF THE MAJOR HISTOCOMPATIBILITY COMPLEX

主要组织相容性复合体的免疫生物学

• 批准号: 3132026
• 负责人: MARCIA M MILLER
• 金额: $ 11.38万
• 依托单位: CITY OF HOPE NATIONAL MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-02-01 至 1988-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3132026
• 关键词: animal population genetics; biological polymorphism; erythroid stem cell; gene expression; genetic library; genetic manipulation; genetic recombination; immune response genes; linkage mapping; major histocompatibility complex; molecular cloning; nucleic acid sequence; surface antigens

The recent findings that many more class I genes may be present within the major histocompatibility complex (MHC) than previously thought, and the emerging evidence that many of these genes encode tissue differentiation antigens, some of which may function in cell recognition outside the immune system, make it appropriate to look more intently at differentiation antigens, some of which may function in cell recognition outside the immune system, make it appropriate to look more intently at differentiation antigens encoded by the MHC. Among these differentiation antigens are highly polymorphic erythroid cell antigens encoded by the chicken MHC. These enigmatic antigens, the B-G antigens, have formed the basis of MHC typing in chickens from the time that the chicken MHC or B system of histocompatibility was first identified. We have purified and analyzed the B-G antigen from the one haplotype, B21, and have found evidence that B-G antigens may be unusual variants of MHC class I molecules. I propose to determine the molecular structure of the B-G 21 antigen in order to better understand the similarities and differences which exist between these polymorphic differentiation antigens and class I transplantation antigens. Microsequencing, tryptic peptide mapping and gene sequencing will be used to determine the structure of the B-G 21 antigen. Genomic and cDNA libraries will be prepared from which the B-G21 antigen gene will be cloned. The B21 class I gene(s) will also be isolated using murine class I gene probes and the sequence of this gene used in the comparative analysis of B-G21. These two genes will be mapped with respect to each other. The low frequency of recombination between them indicates they are closer to each other than many elements within the murine MHC. The basis of the polymorphism of the B-G antigens will be examined using suitable recombinant DNA probes derived from the cloned B-G21 gene. These data will help us to understand the organization and evolution of the MHC.

最近的研究发现，更多的I类基因可能存在于 主要组织相容性复合体（MHC）比以前认为的， 新的证据表明，许多这些基因编码组织分化 抗原，其中一些可能在免疫系统外的细胞识别中起作用 系统，使其更专注于差异化 抗原，其中一些可能在免疫系统外的细胞识别中起作用 系统，使其更专注于差异化 由MHC编码的抗原。 在这些分化抗原中， 由鸡MHC编码的高度多态性红细胞抗原。 这些神秘的抗原，即B-G抗原，形成了MHC的基础。 从鸡的MHC或B系统开始， 首先鉴定了组织相容性。 我们已经提纯并分析了 B-G抗原来自一个单倍型B21，并已发现B-G抗原 抗原可能是MHC I类分子的不寻常变体。 我建议 确定B-G 21抗原的分子结构，以便更好地 了解这些之间存在的相似性和差异 多态性分化抗原和I类移植抗原。 将使用微量测序、胰蛋白酶肽图谱和基因测序 来确定B-G 21抗原的结构 基因组和cDNA 将制备文库，将从所述文库中分离B-G21抗原基因。 克隆的 B21 I类基因也将使用鼠I类基因分离。 基因探针和该基因的序列用于比较分析 关于B-G21 这两个基因将被相互定位。 的 它们之间的重组频率低表明它们更接近于 与鼠MHC内的许多元件不同。 的基础 B-G抗原的多态性将使用合适的 来源于克隆的B-G21基因的重组DNA探针。 这些数据将 帮助我们理解MHC的组织和进化。