EXPRESSION OF FASCIOLA HEPATIC SURFACE ANTIGEN

片形吸虫肝表面抗原的表达

• 批准号: 3134224
• 负责人: ALLISON R FICHT
• 金额: $ 9.82万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-06-01 至 1990-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134224
• 关键词: Schistosoma; Trematoda; animal age group; animal population genetics; bacteriophage lambda; communicable disease diagnosis; developmental genetics; gene expression; genetic library; genetic manipulation; genetic promoter element; genetic recombination; genetic transcription; helminthic antigen; host organism interaction; laboratory rabbit; molecular biology; molecular cloning; monoclonal antibody; nucleic acid probes; nucleic acid sequence; surface antigens

Fasciola hepatica is a digenetic trematode which infects cattle, sheep, and humans and is distributed worldwide. The organism undergoes several dramatic morphological changes three of which occur in the mammalian host and are under investigation in my laboratory. The target of this investigation is the study of stage-specific surface components through the application of the techniques of molecular biology. Surface component coding sequences will be isolated from bacteriophage lambda expression libraries through the use of antibody probes. These cDNA coding sequences will then be used as probes to examine both the genomic location (i.e. gene clustering) and the possible stage-specific transcription of these genes. Copies of coding sequences for the genes will also be isolated from the genome along with flanking sequence in order to examine the promoters and/or enhancers involved in transcription. Very little molecular biology has been carried out on trematode parasites; hopefully this research will serve as a model system for developmental expression of surface antigens in the closely related trematode parasites Schistosoma, Paragonimus and Clonorchis. Finally, a growing body of data suggests that Fasciola, Schistosoma and Paragonimus share antigenic surface components. One such purified surface component from Fasciola has been shown to provide up to 83% protection from subsequent challenge with S. mansoni. These types of data suggest that the protein generated by the recombinant clones in this proposal may be utilized as a component of a vaccine directed generally against trematodes. Alternatively, the proteins produced by these recombinant clones may represent components specific for Fasciola and therefore useful for Fasciola diagnostic tests.

肝片形吸虫是一种复殖吸虫，感染牛、羊和 人，分布在世界各地。 生物体经历了几次 其中三种发生在哺乳动物宿主中 正在我的实验室里接受调查 这次袭击的目标 调查是通过阶段特定的表面成分的研究， 分子生物学技术的应用。 表面部件 将从λ噬菌体表达中分离编码序列 通过使用抗体探针来构建文库。 这些cDNA编码序列 将被用作探针以检查基因组位置（即基因 聚类）和这些基因的可能的阶段特异性转录。 基因的编码序列的拷贝也将从细胞中分离。 基因组沿着与侧翼序列，以检查启动子 和/或参与转录的增强子。 分子生物学 已经对吸虫寄生虫进行了研究;希望这项研究将 作为表面抗原发育表达的模型系统， 密切相关的吸虫寄生虫血吸虫，并殖吸虫和 克隆尼斯。 最后，越来越多的数据表明，片形吸虫、血吸虫和 并殖吸虫共享抗原表面成分。 一个这样的净化表面 来自片形吸虫的成分已被证明可提供高达83%的保护， 与S的后续挑战。mansoni 这些数据表明， 本发明中重组克隆产生的蛋白质可以 用作通常针对以下疾病的疫苗的组分： 吸虫 或者，由这些重组体产生的蛋白质可以是 克隆可以代表片形吸虫特异性的组分， 片形吸虫诊断测试

项目成果

Presclerotized eggshell protein from the liver fluke Fasciola hepatica.

来自肝片形吸虫的预硬化蛋壳蛋白。

• DOI: 10.1021/bi00398a043
• 发表时间: 1987
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Waite,JH;Rice-Ficht,AC
• 通讯作者: Rice-Ficht,AC