EXPRESSION OF FASCIOLA HEPATIC SURFACE ANTIGEN

片形吸虫肝表面抗原的表达

• 批准号: 3134223
• 负责人: ALLISON R FICHT
• 金额: $ 10.41万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-06-01 至 1989-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134223
• 关键词: animal age group; animal population genetics; bacteriophage lambda; communicable disease diagnosis; developmental genetics; gene expression; genetic library; genetic manipulation; genetic recombination; genetic transcription; helminthic antigen; host organism interaction; molecular biology; molecular cloning; monoclonal antibody; nucleic acid sequence; surface antigens

Fasciola hepatica is a digenetic trematode which infects cattle, sheep, and humans and is distributed worldwide. The organism undergoes several dramatic morphological changes three of which occur in the mammalian host and are under investigation in my laboratory. The target of this investigation is the study of stage-specific surface components through the application of the techniques of molecular biology. Surface component coding sequences will be isolated from bacteriophage lambda expression libraries through the use of antibody probes. These cDNA coding sequences will then be used as probes to examine both the genomic location (i.e. gene clustering) and the possible stage-specific transcription of these genes. Copies of coding sequences for the genes will also be isolated from the genome along with flanking sequence in order to examine the promoters and/or enhancers involved in transcription. Very little molecular biology has been carried out on trematode parasites; hopefully this research will serve as a model system for developmental expression of surface antigens in the closely related trematode parasites Schistosoma, Paragonimus and Clonorchis. Finally, a growing body of data suggests that Fasciola, Schistosoma and Paragonimus share antigenic surface components. One such purified surface component from Fasciola has been shown to provide up to 83% protection from subsequent challenge with S. mansoni. These types of data suggest that the protein generated by the recombinant clones in this proposal may be utilized as a component of a vaccine directed generally against trematodes. Alternatively, the proteins produced by these recombinant clones may represent components specific for Fasciola and therefore useful for Fasciola diagnostic tests.

肝片吸虫是一种双基因吸虫，感染牛、羊和 并分布在世界各地。生物体经历了几次 戏剧性的形态变化，其中三种发生在哺乳动物宿主中 正在我的实验室接受调查。这次袭击的目标是 调查是通过对阶段特定表面成分的研究 分子生物学技术的应用。曲面组件 编码序列将从噬菌体lambda表达中分离出来 通过使用抗体探针构建文库。这些cDNA码序列 然后将被用作探针来检测基因组位置(即基因 聚集性)以及这些基因可能的阶段特异性转录。 基因的编码序列的副本也将从 基因组和侧翼序列，以便检查启动子 和/或参与转录的增强子。分子生物学很少 已经在吸虫寄生虫上进行了研究；希望这项研究将 作为发育过程中表面抗原表达的模型系统 关系密切的吸虫寄生虫血吸虫、并殖吸虫和 华支睾吸虫。 最后，越来越多的数据表明，片吸虫、血吸虫和 并殖吸虫具有相同的抗原性表面成分。一个这样的净化表面 来自Fasciola的成分已被证明可提供高达83%的保护 随后对曼氏假单胞菌的挑战。这些类型的数据表明， 本提案中的重组克隆产生的蛋白质可能是 用作一般针对人的疫苗的一种成分 吸虫。或者，由这些重组蛋白产生的蛋白质 克隆可能代表特定于片吸虫的成分，因此很有用 用于片吸虫诊断测试。