Molecular mechanisms of virulence and avirulence in the Avr3a family of Phytophthora.

疫霉Avr3a家族毒力和无毒力的分子机制。

• 批准号: BB/I01957X/1
• 负责人: Mark Banfield
• 金额: $ 51.62万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FI01957X%2F1
• 关键词: Molecular; mechanisms; virulence; avirulence; Avr3a

The oomycetes are fungus-like eukaryotic microorganisms. Several species of the oomycetes are devastating pathogens that are of great importance to world agriculture and food security. In essence, this means that they destroy crops that are critical sources of food. The word 'Phytophthora', which is a genus with the oomycete lineage means 'plant destroyer'. Phytophthora species include the organism responsible for the Irish potato famine, Phytophthora infestans, which causes a disease commonly know as 'potato blight' or 'late blight' (it also causes tomato blight), as well as pepper blight (Phytophthora capsici) and soybean root rot (Phytophthora sojae). Recently, Phytophthora ramorum has gained considerable press in the UK as it is threatening iconic trees, such as oak. Phytophthora infestans remains the best-known and, arguably, the most important oomycete pathogen. It continues to cost modern agriculture billions of pounds annually and also impacts subsistence farming in developing countries. With potato now ranked the third most important crop in the world, P. infestans is an important biotic threat to global food security. Damage caused by other Phytophthora species is also severe, on a global scale. Our long-term objective is to understand how oomycetes, particularly Phytophthora, successfully infect plants and dissect the plant processes that are affected by these pathogens. It is now well established that, like other pathogens, Phytophthora species secrete a number of proteins, termed 'effectors', that modulate the immune response of plants and enable host colonization. Deciphering the biochemical activities of effectors is critical to understanding mechanisms of pathogenesis. Among these proteins, RxLR-type effectors, named after an amino acid sequence present in the protein, are targeted to the inside of plant cells. In this proposal, we focus on a particular group of RxLR effectors that is found in the Phytophthora species mentioned above. These are all members of the Avr3a family, as they share amino acid sequence homology to the Phytophthora infestans effector Avr3a. Building on preliminary data we have already obtained, we aim to define what conserved and divergent functions these proteins have in suppressing the plant immune system. Through host factors, members of this protein family can also initiate a programmed cell-death response in plants designed to limit pathogen growth. We also aim to understand some details of how early steps in this response occur, specifically how these effectors are detected by host cell factors. This study will help to establish functional connections between pathogen proteins and plant processes. Detailed knowledge of how the Avr3a family is able to manipulate certain aspects of plant immunity and be recognized by others will improve our understanding of the infection process and enable novel strategies for engineering resistance to Phytophthora pathogens.

卵菌是真菌样的真核微生物。卵菌的几个物种是毁灭性的病原体，对世界农业和粮食安全非常重要。从本质上讲，这意味着它们破坏了作为重要食物来源的作物。“Phytophthora”这个词是卵菌谱系的一个属，意思是“植物破坏者”。疫霉属物种包括导致爱尔兰马铃薯饥荒的生物体，致病疫霉，其引起通常称为“马铃薯枯萎病”或“晚疫病”的疾病（它也引起番茄枯萎病），以及辣椒枯萎病（辣椒疫霉）和大豆根腐病（大豆疫霉）。最近，Phytophthora ramorum在英国获得了相当大的新闻，因为它正在威胁橡树等标志性树木。致病疫霉仍然是最著名的，可以说是最重要的卵菌病原体。它继续每年使现代农业损失数十亿英镑，并影响发展中国家的自给农业。马铃薯现在是世界上第三大作物，致病疫霉是全球粮食安全的重要生物威胁。在全球范围内，其他疫霉属物种造成的损害也很严重。我们的长期目标是了解卵菌，特别是疫霉，如何成功地感染植物，并解剖受这些病原体影响的植物过程。现在已经确定，与其他病原体一样，疫霉属物种分泌许多蛋白质，称为“效应子”，其调节植物的免疫应答并使宿主定殖成为可能。解读效应子的生化活性对于理解发病机制至关重要。在这些蛋白质中，RxLR型效应子以蛋白质中存在的氨基酸序列命名，靶向植物细胞内部。在这个建议中，我们专注于一组特定的RxLR效应子，发现在上述疫霉属物种。这些都是Avr3a家族的成员，因为它们与致病疫霉效应子Avr3a共享氨基酸序列同源性。在我们已经获得的初步数据的基础上，我们的目标是确定这些蛋白质在抑制植物免疫系统中具有哪些保守和不同的功能。通过宿主因素，该蛋白质家族的成员也可以在植物中启动程序性细胞死亡反应，以限制病原体的生长。我们还旨在了解这种反应的早期步骤如何发生的一些细节，特别是这些效应子如何被宿主细胞因子检测到。该研究将有助于建立病原蛋白与植物过程之间的功能联系。详细了解Avr3a家族如何能够操纵植物免疫的某些方面并被其他人识别，将提高我们对感染过程的理解，并使工程抗疫霉病原体的新策略成为可能。