IMMUNOREGULATION BY ALLERGEN-SPECIFIC T-CELL CLONES

过敏原特异性 T 细胞克隆的免疫调节

• 批准号: 3138566
• 负责人: Lanny J. Rosenwasser
• 金额: $ 18.23万
• 依托单位: NATIONAL JEWISH HEALTH
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3138566
• 关键词: T cell receptor; T lymphocyte; adult human (21+); allergens; antibody titering; antigens; clone cells; flow cytometry; genetic manipulation; helper T lymphocyte; histocompatibility antigens; human tissue; hypersensitivity desensitization; immunoregulation; interleukin 1; laboratory mouse; leukocyte activation /transformation; lymphokines; major histocompatibility complex; monoclonal antibody; nucleic acid sequence; protein sequence; radioimmunoassay; suppressor T lymphocyte; synthetic peptide; tissue /cell culture

The interaction of allergen with immune cells, in particular thymus derived lymphocytes and antigen presenting cells, is critical to the development of allergy in humans. The goals of this proposal involve an examination of the specificity of interaction between the antigenic epitopes of allergen derived from murine tissues, antigen presenting cells and specific immune T cells derived from individuals allergic to laboratory mice. The direction of these studies will be focused on the development of allergen specific human T cell clones and then an in depth characterization of these clones in terms of the structure of the allergen recognized by T cells in association with class II major histocompatibility complex antigens and the requirements for activation for the T cell clones. These human T cell clones will also be studied for their potential immunoregulatory effects in vitro and for their ability to moderate the production of specific immunoglobulin by allergen specific B lymphocytes. In addition, the potential contribution of specific allergen structure to the specificity and activation of the human T cell clones will be examined via the generation of peptide fragments related to primary structure of the putative mouse allergen derived from concentrated murine urine. A better understanding of the interaction of allergen with accessory cells and immune T cells will provide new and unique ways to potentially regulate specific immune responses and this may be of considerable value in the immunotherapy of allergic disease and in fact in the therapy of immune base diseases in general.

过敏原与免疫细胞的相互作用，特别是 胸腺来源的淋巴细胞和抗原呈递细胞， 对人类过敏的发展至关重要。 的目标 这一建议涉及审查以下方面的具体情况： 过敏原衍生的抗原表位之间的相互作用 从鼠组织、抗原呈递细胞和特异性免疫 T细胞来源于对实验室小鼠过敏的个体。 的 这些研究的方向将集中在发展 过敏原特异性人类T细胞克隆，然后深入研究 这些克隆的结构方面的表征 T细胞识别的过敏原与II类主要 组织相容性复合物抗原和对 激活T细胞克隆。 这些人类T细胞克隆将 还可以研究它们在以下方面的潜在免疫调节作用： 体外和他们的能力，以缓和生产的具体 免疫球蛋白通过过敏原特异性B淋巴细胞。 此外，本发明还提供了一种方法， 特定过敏原结构对过敏原的潜在贡献 人T细胞克隆的特异性和活化将是 通过产生与以下相关的肽片段进行检查： 小鼠过敏原的一级结构 浓缩的鼠尿。 更好地理解 过敏原与辅助细胞和免疫T细胞相互作用 将提供新的和独特的方式来潜在地监管特定的 免疫反应，这可能是相当大的价值， 过敏性疾病的免疫治疗， 免疫基础疾病。

项目成果

Allergen-specific human T cell clones: derivation, specificity, and activation requirements.

过敏原特异性人类 T 细胞克隆：衍生、特异性和激活要求。

• DOI: 10.1016/0091-6749(89)90110-3
• 发表时间: 1989
• 期刊: The Journal of allergy and clinical immunology
• 作者: Gurka,G;OhmanJr,J;Rosenwasser,LJ
• 通讯作者: Rosenwasser,LJ