BIOCHEMICAL MECHANISMS IN INSECT IMMUNITY

昆虫免疫的生化机制

• 批准号: 3136998
• 负责人: A J NAPPI
• 金额: $ 12.12万
• 依托单位: LOYOLA UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3136998
• 关键词: Drosophilidae; Hymenoptera; catecholamines; chemical structure function; cytology; decarboxylases; electrochemistry; enzyme induction /repression; enzyme mechanism; enzyme substrate; genetic strain; hemolymph; host organism interaction; humoral immunity; immunoregulation; immunosuppression; intracellular parasitism; oxidoreductase; scintillation counter; spectrometry; statistics /biometry

The proposed research is designed to provide important information on the cytological an biochemical bases of immune recognition and regulation in insects. Experiments are designed using specific enzyme assays, sensitive electrochemical methods and lectin binding specificities, to assess the roles of blood cells and hemolymph components in the synthesis and utilization of substrate(s), enzymes and various intermediate catecholamine derivatives that form protein-polyphenol complexes around metazoan parasites. The array of genetically variant enzyme mutants of Drosophila melanolgaster hosts, together with both susceptible and resistant strains of the wasp parasitoid Leptopilina heterotoma available for the proposed study provide exceptionally suitable experimental systems for investigating not only the cell-mediated immune response that is manifested by numerous insects, but also the mechanisms of parasite-induced suppression of host defenses.

拟议的研究旨在提供有关 免疫识别和调节的细胞学和生物化学基础， 昆虫 实验设计使用特定的酶测定，灵敏度 电化学方法和凝集素结合特异性，以评估 血细胞和血淋巴成分在合成和 底物、酶和各种中间体儿茶酚胺的利用 在后生动物周围形成蛋白质-多酚复合物的衍生物 寄生虫 果蝇的遗传变异酶突变体阵列 melanolgaster宿主，以及敏感和抗性菌株 的寄生蜂Leptopilina heterotoma可用于建议 研究提供了非常适合研究的实验系统 不仅细胞介导的免疫反应， 昆虫，而且寄生虫引起的抑制宿主的机制 的防御合作.