BIOLOGY OF LENTIVIRUS SURFACE CARBOHYDRATES

HIV和动物慢病毒是中和的不良诱导剂 在感染期间或在免疫实验中的抗体 动物。此外，中和抗体在治疗中的有效性 预防艾滋病毒感染很难评估，因为 缺乏关于抗体亲和力差异的数据 病毒和宿主细胞的病毒。关于中和的研究 动物慢病毒表明抗体之间的亲和力 而且病毒的含量很低。更重要的是，最贫穷的病毒 本课程介绍了中和安托贝德的诱导剂和反应堆。 有一层密集的碳水化合物。我们的假设是病毒 表面碳水化合物是诱导和反应的关键。 用于中和抗体和与细胞结合。初步数据 已经表明神经氨酸酶对 病毒。使用病毒的各种底物，病毒感染的细胞 和脱糖病毒感染细胞来吸收免疫血清；我们 将决定病毒表面碳水化合物是否是主要的 病毒中和中的表位。我们还将确定是否 病毒的碳水化合物是病毒与病毒结合的关键 细胞和后来对细胞的渗透。最后，我们将表演一个 对病毒碳水化合物的结构进行详细分析 关于两种原型病毒的O-和N-连接糖基化 它们的生物学特性差异很大。

HIV and the animal lentiviruses are poor inducers of neutralizing antibodies either during infection or in immunized experimental animals. Further, the effectiveness of neutralizing antibodies in protecting against HIV Infection is difficult to evaluate because of lack of data on differences between the affinity of antibodies for the virus and virus for the host cell. Studies on neutralization of the animal lentiviruses show that affinity between antibodies and virus is very low. Morevoer, viruses which are the poorest inducers of, and reactors with, neutralizing antobides are covered with a dense layer of carbohydrates. Our hypothesis is that viral surface carbohydrates are crucial for inducing and reacting with neutralizing antibodies and for binding to cells. Preliminary data already show that neuraminidase has major biological effects on the viruses. Using various substrates of virus, virus-infected cells and deglycosylated virus-infected cells to absorb immune sera; we will determine whether virus surface carbohydrates are the major epitopes in virus neutralization. We will also determine whether the viral carbohydrates are essential for binding of the virus to cells and later penetration of the cells. Finally, we will perform a detailed analysis of the structure of the viral carbohydrates with respect to O- and N-linked glycosylation on two prototype viruses that vary widely in biological properties.