HUMAN ANTI-H. INFLUENZAE B IDIOTYPES

尽管有抗生素，流感嗜血杆菌B 仍然是儿童发病和死亡的主要原因。 本研究旨在分析人类个体的独特型多样性， 主要抗原和毒力因子特异性抗体 H.流感病毒B，荚膜碳水化合物，多核糖基-核糖醇- 磷酸盐（PRP）。 使用杂交瘤方法， 特异于交叉反应独特位的单克隆抗体 将制备PRP特异性抗体。 这些反白痴 试剂将用于确定：独特位 在不相关的人类之间共享，无论是婴儿还是成年人， 表达相似的独特型谱系，以及是否抗独特位 抗体可以使新生大鼠对H. 流感B型感染。 此外，还将尝试识别 同种异体人抗独特型抗体。 这些研究将 提供了深入了解可变区多样性和个体发育的 人抗PRP抗体库以及测试 使用抗独特型抗体作为保护性H. 流感B疫苗。 人类独特型的鉴定和抗- 独特型是必要的，为未来的研究，寻求确定 抗独特型抗体是否调节B细胞表达， 婴儿和成人。

Despite the availability of antibiotics, Hemophilus influenzae b remains a leading cause of morbidity and mortality in children. This study proposes to analyze the idiotypic diversity of human antibodies specific for the major antigen and virulence factor of H. influenzae b, the capsular carbohydrate, polyribosyl-ribitol- phosphate (PRP). Using hybridoma methodology, murine monoclonal antibodies specific from cross-reactive idiotopes of PRP-specific antibodies will be prepared. These anti-idiotopic reagents will be used to determine: the extent to which idiotopes are shared between unrelated humans, whether infants and adults express similar idiotypic repertoires, and whether anti-idiotopic antibodies can prime neonatal rats for protection from H. influenzae b infection. Also, attempts will be made to identify isologous human anti-idiotypic antibodies. These studies will provide insight into the variable region diversity and ontogeny of the human anti-PRP antibody repertoire as well as test the feasibility of using anti-idiotypic antibodies as a protective H. influenzae b vaccine. Identification of human idiotypes and anti- idiotypes is necessary for future studies which seek to determine whether anti-idiotypic antibodies regulate B cell expression in the infant and the adult.