MOLECULAR BASIS OF PERSISTENT VIRUS INFECTION

持续病毒感染的分子基础

• 批准号: 3138619
• 负责人: PETER J SOUTHERN
• 金额: $ 11.67万
• 依托单位: SCRIPPS CLINIC AND RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3138619
• 关键词: DNA directed RNA polymerase; defective virus; disease /disorder model; gel electrophoresis; gene expression; genetic manipulation; immunoregulation; interfering virus; lymphocytic choriomeningitis; molecular pathology; virus RNA; virus cytopathogenic effect; virus infection mechanism

Virus infections often lead to pathogenic consequences and disease considerably beyond the acute phase on infection. The significance of persistent infections for human disease processes is only now becoming apparent. In the course of a persistent infection, disease may develop as a consequence of host immune responses against the virus and virus infected cells and/or from virus disruption of cellular functions. At present, there is only limited understanding of the molecular details of persistent virus infection. There have been many suggestions that defective interfering (DI) RNAs may be involved with the establishment and maintenance of persistent virus infections. Although DI RNAs have been linked to persistence in tissue culture systems, there is little information that relates directly to the appearance and function of DI RNAs during infection in vivo. The studies proposed here will examine persistent infections induced in laboratory mice by lymphocytic choriomeningitis virus to determine whether deleted RNAs (i.e., potential DI RNAs) appear and accumulate in persistently infected animals and whether these RNAs alter the normal course of acute infection. A complementary series of experiments will investigate potential regulatory mechanisms that reduce viral gene expression and may mediate the transition from acute to persistence infection. A detailed understanding of the molecular basis of virus persistence in one system will allow conceptual developments for the whole field of virus persistence and may suggest strategies for intervention and eventual clearance of a persistent infection.

病毒感染常常导致致病性的后果