MOLECULAR BASIS OF PERSISTENT VIRUS INFECTION

随着检测方法变得越来越复杂，有越来越多的 意识到病毒可以驻留在表面上正常的宿主中 延长期限。在这种长期感染期间，病毒重新激活可能 自发发生或在小鼠免疫状态改变后发生 主持人。病毒复制的开始可能通过以下方式启动致病事件 激发对病毒和病毒感染细胞的免疫反应 和/或病毒对细胞功能的破坏。的重要意义。 人类疾病过程中的长期感染直到现在才完全 显然，有几种说法表明，病毒可能是 与慢性退行性疾病有关。目前，只有 对与之相关的分子事件的了解有限 建立和维持长期的病毒感染，包括 关于病毒基因表达的变化和病毒引起的变化 在感染者体内。我打算研究一个性格特点很好的 基于实验室持续感染的可复制模型系统 小鼠和组织培养细胞感染淋巴细胞性脉络膜脑膜炎病毒 确定促成从急性到 持续性感染和随后的持续性维护 感染。对病毒分子基础的详细解释 在一个系统中的持久化将允许对整个系统进行概念开发 病毒持久性领域，并可能建议干预和 持续感染的最终清除。

As detection methods become more sophisticated, there is an increasing realization that viruses can reside within apparently normal hosts for extended periods. During such long-term infections, virus reactivation may occur spontaneously or after changes in the immunological status of the host. The onset of virus replication may initiate pathogenic events by provoking immune responses against the virus and virus infected cells and/or from virus disruption of cellular functions. The significance of long-term infections for human disease processes is only now becoming fully apparent and there have been several suggestions that viruses may be involved with chronic degenerative diseases. At present, there is only limited understanding of the molecular events associated with the establishment and maintenance of long-term virus infections both with respect to alterations in virus gene expression and virus-induced changes within the infected individual. I intend to study a well-characterized and reproducible model system, based on persistent infections of laboratory mice and tissue culture cells with lymphocytic choriomeningitis virus, to identify regulatory events that mediate the transition from acute to persistent infection and subsequent maintenance of the persistent infection. A detailed explanation for the molecular basis of virus persistence in one system will allow conceptual developments for the whole field of virus persistence and may suggest strategies for intervention and eventual clearance of a persistent infection.