MOLECULAR GENETICS OF GEMINIVIRUSES SQLCV AND MSV

该研究的重点是基因的分子分析， 在引起南瓜曲叶病的病毒中表达（SqLCV- NR和SqLCV-BR）和玉米条斑病（MSV）。 这些研究 他们的主要目标是绘制基因组的功能图 目的：（1）鉴定病毒基因组元件 在寄主范围和致病性方面具有重要意义;（2）明确了致病因子 基因表达所需的，特别是复制和 转录，在这些病毒中， 作为双生病毒基因表达的模型， 单子叶植物和双子叶植物;（3）开始鉴定细胞因子 在植物基因调控中起重要作用。 生物测定， 农杆菌接种的表达，紧密相关的序列元件， 相关的SqLCV-NR和SqLCV-BR，它们具有不同的宿主范围， 将被交换，并研究突变体的宿主范围 表型和引起全身性疾病的能力。 MSV删除和 还将分析基因置换突变体的能力， 感染单子叶植物或双子叶植物。 这些将通过以下方式进行调查： 突变体分析，并因此鉴定序列元件，它们 将用于结合试验，以鉴定细胞和病毒因子 参与基因调控。 除了病原体， 研究将增加我们对宿主的基本机制的理解， 范围限制、致病机制和真核基因表达。

The proposed research focuses on the molecular analysis of gene expression in the viruses causing squash leaf curl disease (SqLCV- NR and SqLCV-BR) and maize streak disease (MSV). These studies have as their primary goal the functional mapping of the genomes of SqLCV and MSV in order to: (1) identify viral genomic elements important in host range and pathogeneses; (2) define the elements required for gene expression, specifically replication and transcription, in these viruses in an effort to understand regulation in geminiviruses and as a model for gene expression in monocots and dicots; and, (3) begin to identify cell factors important in gene regulation in plants. Biological assays for the expression of agroinoculation, sequence elements of the closely related SqLCV-NR nd SqLCV-BR, which have different host ranges, will be exchanged and mutants studied for their host range phenotypes and ability to cause systemic disease. MSV deletion and gene substitution mutants will also be analyzed for their ability to infect monocots or dicots. These will be investigated through mutant analysis and as such sequence elements are identified they will be used in binding assay to identify cell and viral factors involved in gene regulation. In addition to pathogens, these studies will add to our understanding of basic mechanisms of host range restriction, pathogenesis and eukaryotic gene expression.