MOLECULAR GENETICS OF GEMINIVIRUSES SQLCV AND MSV

双生病毒 SQLCV 和 MSV 的分子遗传学

• 批准号: 3141666
• 负责人: SONDRA G LAZAROWITZ
• 金额: $ 15.58万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3141666
• 关键词: gene expression; genetic manipulation; genetic mapping; genetic transcription; molecular cloning; mutant; nucleic acid sequence; plant diseases; plant genetics; plant virus; transcription factor; virus DNA; virus genetics; virus infection mechanism; virus replication

The proposed research focuses on the molecular analysis of gene expression in the viruses causing squash leaf curl disease (SqLCV- NR and SqLCV-BR) and maize streak disease (MSV). These studies have as their primary goal the functional mapping of the genomes of SqLCV and MSV in order to: (1) identify viral genomic elements important in host range and pathogeneses; (2) define the elements required for gene expression, specifically replication and transcription, in these viruses in an effort to understand regulation in geminiviruses and as a model for gene expression in monocots and dicots; and, (3) begin to identify cell factors important in gene regulation in plants. Biological assays for the expression of agroinoculation, sequence elements of the closely related SqLCV-NR nd SqLCV-BR, which have different host ranges, will be exchanged and mutants studied for their host range phenotypes and ability to cause systemic disease. MSV deletion and gene substitution mutants will also be analyzed for their ability to infect monocots or dicots. These will be investigated through mutant analysis and as such sequence elements are identified they will be used in binding assay to identify cell and viral factors involved in gene regulation. In addition to pathogens, these studies will add to our understanding of basic mechanisms of host range restriction, pathogenesis and eukaryotic gene expression.

拟开展的研究重点是基因的分子分析