MOLECULAR GENETICS OF GEMINIVIRUSES SQLCV AND MSV

建议的研究重点是基因的分子分析。 南瓜曲叶病毒(SqLCV-SqLCV-1)的表达 NR和SqLCV-BR)和玉米条纹叶枯病(MSV)。这些研究 把基因组的功能图谱作为他们的主要目标 SqLCV和MSV的比较，以便：(1)鉴定病毒基因组元件 在寄主范围和致病机制中很重要；(2)定义元素 基因表达所必需的，特别是复制和 转录，在这些病毒中努力了解 双生病毒的调控和作为基因表达的模型 单子叶和双子叶；以及，(3)开始鉴定细胞因子 在植物的基因调控中很重要。生物检验法 表达的农用接种法，序列元件的紧密结合 具有不同寄主范围的相关SqLCV-NR和SqLCV-BR， 将被交换并研究它们的寄主范围的突变体 表型和引起系统性疾病的能力。MSV删除和 基因替代突变体也将被分析其能力。 感染单子叶植物或双子叶植物这些问题将通过以下途径进行调查 突变分析和这样的序列元素被鉴定为 将用于结合分析以识别细胞和病毒因子 参与基因调控。除了病原体，这些 研究将增加我们对寄主的基本机制的理解 范围限制、发病机制和真核基因表达。

The proposed research focuses on the molecular analysis of gene expression in the viruses causing squash leaf curl disease (SqLCV- NR and SqLCV-BR) and maize streak disease (MSV). These studies have as their primary goal the functional mapping of the genomes of SqLCV and MSV in order to: (1) identify viral genomic elements important in host range and pathogeneses; (2) define the elements required for gene expression, specifically replication and transcription, in these viruses in an effort to understand regulation in geminiviruses and as a model for gene expression in monocots and dicots; and, (3) begin to identify cell factors important in gene regulation in plants. Biological assays for the expression of agroinoculation, sequence elements of the closely related SqLCV-NR nd SqLCV-BR, which have different host ranges, will be exchanged and mutants studied for their host range phenotypes and ability to cause systemic disease. MSV deletion and gene substitution mutants will also be analyzed for their ability to infect monocots or dicots. These will be investigated through mutant analysis and as such sequence elements are identified they will be used in binding assay to identify cell and viral factors involved in gene regulation. In addition to pathogens, these studies will add to our understanding of basic mechanisms of host range restriction, pathogenesis and eukaryotic gene expression.