Micro-patterned polymer substrates as novel models of epidermal wound healing

微图案聚合物基质作为表皮伤口愈合的新模型

• 批准号: BB/J000914/1
• 负责人: John Connelly
• 金额: $ 40.95万
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ000914%2F1
• 关键词: Micro; patterned; polymer; substrates; novel

The proposed research will develop a novel in vitro model of wound healing within the skin. We will employ state-of-the-art micro-fabrication techniques to create engineered substrates for culturing human keratinocytes. This technology will allow us to precisely control multiple parameters of the wound environment. Specifically, we will determine how the composition, geometry, and stiffness of the wound influence cell behaviour. In addition, we will examine the molecular signalling pathways that regulate the cellular responses to these different environments. This project has the potential to provide significant insights into how human skin cells sense and respond to extrinsic signals during the wound healing process. Moreover, this model system may be a powerful tool for future cell biology studies, drug screening, and other types of translational research.

这项拟议的研究将开发一种新的皮肤内伤口愈合的体外模型。我们将采用最先进的微制造技术来创造培养人类角质形成细胞的工程基板。这项技术将使我们能够精确控制伤口环境的多个参数。具体来说，我们将确定伤口的组成，几何形状和刚度如何影响细胞行为。此外，我们将研究调节细胞对这些不同环境的反应的分子信号通路。该项目有可能为人类皮肤细胞在伤口愈合过程中如何感知和响应外部信号提供重要见解。此外，该模型系统可能是未来细胞生物学研究、药物筛选和其他类型转化研究的有力工具。

项目成果

Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes.

• DOI: 10.1038/srep33893
• 发表时间: 2016-09-21
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Sharili AS;Kenny FN;Vartiainen MK;Connelly JT
• 通讯作者: Connelly JT

Biophysical signals controlling cell fate decisions: How do stem cells really feel?

• DOI: 10.1016/j.biocel.2012.09.003
• 发表时间: 2012-12-01
• 期刊: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
• 影响因子: 4
• 作者: Costa, Patricia;Almeida, Filipe V. M.;Connelly, John T.
• 通讯作者: Connelly, John T.

High-Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform.

微图案筛选平台内细胞迁移动力学的高内涵分析。

• DOI: 10.1002/adbi.201900011
• 发表时间: 2019
• 期刊: Advanced biosystems
• 影响因子: 4.1
• 作者: Almeida FV