INSECT ADIPOKINETIC HORMONE GENE EXPRESSION

昆虫脂肪运动激素基因表达

• 批准号: 3142852
• 负责人: JAMES Y BRADFIELD
• 金额: $ 14.97万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3142852
• 关键词: biotechnology; chemical synthesis; complementary DNA; gene expression; genetic manipulation; genetic transcription; genetic translation; high performance liquid chromatography; immunological substance; insect virus; laboratory rabbit; molecular cloning; molecular genetics; neurohormones; nucleic acid hybridization; nucleic acid sequence; peptide hormone; protein sequence; radioimmunoassay; radiotracer; structural genes; tissue /cell culture; transposon /insertion element; virus genetics

Peptide hormones (e.g. insulin, growth hormone among hundreds) regulate many aspects of the life cycle of all animals. Understanding how these molecules become available for function requires knowledge of the steps of their synthesis. With modern techniques of molecular biology and chemistry, the pathways of hormone synthesis can now be elucidated. Peptide hormone synthesis in insects, a group of animals containing many species of great importance to human health, is unfortunately very poorly understood. It is proposed that knowledge of insect hormone gene expression can lead to environmentally safe means of disrupting the physiological balance of injurious species. The adipokinetic hormone (AKH) family of insect neurohormones is the largest group of related peptides yet described from nature, and likely is represented in all insect types. One of the major goals of this proposed research is to characterize AKH genes and gene products. The research will give new information on physiological regulation in one of the most important groups of animals. Use of genetic engineering to produce proteins of medical importance (e.g. vaccines) is one of the important promises of biotechnology. one of the widely accepted systems for production of useful proteins is an insect baculovirus expression vector system developed here at Texas A&M University. Much needs to be learned of factors affecting the efficiency of foreign gene expression by the baculovirus. Analysis of the efficiency of insect gene (AKH) expression directed by the insect-specific baculovirus may indicate strategies for improved production of proteins valuable to man.

多肽激素(如胰岛素，数百种生长激素)调节 所有动物生命周期的许多方面。了解这些功能是如何 分子可用于功能需要了解以下步骤 它们的合成。利用现代分子生物学和化学技术， 激素合成的途径现在可以阐明了。 昆虫中的多肽激素合成，一群包含许多 对人类健康非常重要的物种，不幸的是非常差 明白了。认为对昆虫激素基因表达的认识 会导致对环境安全的方式来破坏生理 有害物种的平衡。 昆虫神经激素中的脂肪运动激素(AKH)家族是 迄今从自然中描述的最大一组相关多肽，很可能是 在所有昆虫类型中都有表现。这项提议的主要目标之一是 研究是为了确定AKH基因和基因产物的特征。这项研究将 给出了关于生理调节的新信息 重要的动物群体。 利用基因工程来生产具有医学意义的蛋白质(例如 疫苗)是生物技术的重要承诺之一。其中一个 被广泛接受的生产有用蛋白质的系统是昆虫 德克萨斯A&M公司开发的杆状病毒表达载体系统 大学。需要了解许多影响工作效率的因素 杆状病毒的外源基因表达。对企业资源配置效率的分析 昆虫特异性杆状病毒介导的昆虫基因(AKH)表达 可能表明提高有价值的蛋白质生产的策略 天哪。