INSECT ADIPOKINETIC HORMONE GENE EXPRESSION

昆虫脂肪运动激素基因表达

• 批准号: 3142853
• 负责人: JAMES Y BRADFIELD
• 金额: $ 15.92万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3142853
• 关键词: biotechnology; chemical synthesis; complementary DNA; gene expression; genetic manipulation; genetic transcription; genetic translation; high performance liquid chromatography; immunological substance; insect virus; laboratory rabbit; molecular cloning; molecular genetics; neurohormones; nucleic acid hybridization; nucleic acid sequence; peptide hormone; protein sequence; radioimmunoassay; radiotracer; structural genes; tissue /cell culture; transposon /insertion element; virus genetics

Peptide hormones (e.g. insulin, growth hormone among hundreds) regulate many aspects of the life cycle of all animals. Understanding how these molecules become available for function requires knowledge of the steps of their synthesis. With modern techniques of molecular biology and chemistry, the pathways of hormone synthesis can now be elucidated. Peptide hormone synthesis in insects, a group of animals containing many species of great importance to human health, is unfortunately very poorly understood. It is proposed that knowledge of insect hormone gene expression can lead to environmentally safe means of disrupting the physiological balance of injurious species. The adipokinetic hormone (AKH) family of insect neurohormones is the largest group of related peptides yet described from nature, and likely is represented in all insect types. One of the major goals of this proposed research is to characterize AKH genes and gene products. The research will give new information on physiological regulation in one of the most important groups of animals. Use of genetic engineering to produce proteins of medical importance (e.g. vaccines) is one of the important promises of biotechnology. one of the widely accepted systems for production of useful proteins is an insect baculovirus expression vector system developed here at Texas A&M University. Much needs to be learned of factors affecting the efficiency of foreign gene expression by the baculovirus. Analysis of the efficiency of insect gene (AKH) expression directed by the insect-specific baculovirus may indicate strategies for improved production of proteins valuable to man.

肽激素（例如胰岛素、生长激素等数百种）调节 所有动物生命周期的许多方面。了解这些如何 分子变得可发挥功能需要了解以下步骤 他们的合成。借助现代分子生物学和化学技术， 现在可以阐明激素合成的途径。 昆虫中肽激素的合成，这是一组含有许多肽的动物 对人类健康非常重要的物种，不幸的是非常糟糕 明白了。建议了解昆虫激素基因表达 可以导致破坏生理的环境安全的方法 有害物种的平衡。 昆虫神经激素中的脂肪运动激素 (AKH) 家族是 迄今为止从自然界中描述的最大一组相关肽，并且很可能是 代表所有昆虫类型。该提议的主要目标之一 研究的目的是表征 AKH 基因和基因产物。该研究将 提供有关生理调节的新信息之一 重要的动物类群。 利用基因工程生产具有医学重要性的蛋白质（例如 疫苗）是生物技术的重要前景之一。中的一个 广泛接受的生产有用蛋白质的系统是昆虫 德克萨斯 A&M 开发的杆状病毒表达载体系统 大学。影响效率的因素有很多需要了解 杆状病毒表达外源基因。效率分析 由昆虫特异性杆状病毒指导的昆虫基因 (AKH) 表达 可能表明改善有价值的蛋白质生产的策略 男人。