Bilateral BBSRC-FAPESP: Cellular and regulatory basis for early plant organ growth

双边 BBSRC-FAPESP：早期植物器官生长的细胞和调控基础

• 批准号: BB/J007056/1
• 负责人: Robert Sablowski
• 金额: $ 59.05万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ007056%2F1
• 关键词: Bilateral; BBSRC; FAPESP; Cellular; regulatory

A central problem in Biology is to understand how genes cause organs to grow to a specific shape and size. Plants are convenient to address this question because their overall growth results primarily from the increase in cell numbers and increase in the size of individual cells (cell movement and "pruning" by cell death do not need to be considered). In addition, understanding organ growth in plants offers a clear path to practical use through the rational manipulation of crop growth and yield. A major bottleneck for understanding plant growth, however, is that although we know several genes that control the overall size and shape of organs, we do not understand what processes these genes control within cells (such as cell division or increase in cell mass) to result in a net effect on the total size and shape of organs. We aim to answer this question, by studying the early stages of floral organ development in the model species, Arabidopsis. Unprecedented opportunities to address the question above arise from two recent developments. One is the establishment of methods that allow quantitative, 3D analysis of cell geometry and cell division in growing organs. Using these methods, the UK partner in this project has recently found that a key regulator of organ growth, called JAGGED (JAG), has an unanticipated role in co-ordinating cell volume with cell division in developing organs. The second is the development of techniques for detection of all genes controlled by a given regulatory gene, at well-defined stages of organ formation - this can reveal the repertoire of cellular functions that are controlled by a regulatory gene. Our Brazilian/Dutch partners have been developing these methods and applying them to understand the role of genes that control floral organ development. Taking advantage of the complementary expertise, resources and biological interest of the UK and Brazilian/Dutch partners, we will extend both approaches to a key set of genes that control plant organ growth: JAGGED (JAG), AINTEGUMENTA (ANT) and CIN-TCP genes. We will test whether the co-ordination between cell size and cell division is a key feature of targeted by these genes at the early stages of organ growth. We will also test whether these genes target specific steps in cell division and clarify how the activities of these regulatory genes are combined during organ growth. Finally, we will identify the sets of genes controlled by JAG and CIN-TCP genes in the early stages of organ development - this will show to what extent the function of these genes overlap, and reveal the key cellular functions targeted by these genes to determine how the organs grow. One of the current priorities in developmental biology is to develop computer models that can simulate and predict the way organs and organisms grow. Addressing the questions in this proposal will be essential for connecting these models with molecular mechanisms that can be controlled experimentally. Ultimately, this knowledge will allow rational modification of plant organ growth, which has an obvious impact on crop performance. In addition to addressing a fundamental biological question with strategic relevance, our work will establish new and mutually beneficial long-term scientific links between UK and Brazilian institutions.

生物学的一个核心问题是了解基因如何使器官生长到特定的形状和大小。植物很容易解决这个问题，因为它们的整体生长主要是由于细胞数量的增加和单个细胞大小的增加（细胞运动和细胞死亡的“修剪”不需要考虑）。此外，了解植物器官的生长提供了一个明确的途径，通过合理操纵作物生长和产量的实际应用。然而，理解植物生长的一个主要瓶颈是，尽管我们知道几个控制器官整体大小和形状的基因，但我们不知道这些基因控制细胞内的哪些过程（如细胞分裂或细胞质量的增加），从而对器官的整体大小和形状产生净效应。我们的目标是回答这个问题，通过研究模式物种，拟南芥花器官发育的早期阶段。最近的两个事态发展为解决上述问题提供了前所未有的机会。一个是建立方法，允许定量，3D分析生长器官中的细胞几何形状和细胞分裂。利用这些方法，该项目的英国合作伙伴最近发现，一种名为JAGGED（JAG）的器官生长关键调节因子在协调细胞体积与器官发育中的细胞分裂方面具有意想不到的作用。第二个是在器官形成的明确阶段，开发检测由给定调控基因控制的所有基因的技术-这可以揭示由调控基因控制的细胞功能库。我们的巴西/荷兰合作伙伴一直在开发这些方法，并将其应用于了解控制花器官发育的基因的作用。利用英国和巴西/荷兰合作伙伴的互补专业知识，资源和生物学兴趣，我们将把这两种方法扩展到控制植物器官生长的一组关键基因：JAGGED（JAG），AINTEGUMENTA（ANT）和CIN-TCP基因。我们将测试细胞大小和细胞分裂之间的协调是否是这些基因在器官生长早期阶段靶向的关键特征。我们还将测试这些基因是否靶向细胞分裂中的特定步骤，并阐明这些调控基因的活动在器官生长过程中是如何结合的。最后，我们将确定在器官发育的早期阶段由JAG和CIN-TCP基因控制的基因组-这将显示这些基因的功能重叠的程度，并揭示这些基因靶向的关键细胞功能，以确定器官如何生长。发育生物学目前的优先事项之一是开发能够模拟和预测器官和生物体生长方式的计算机模型。解决本提案中的问题对于将这些模型与可以通过实验控制的分子机制联系起来至关重要。最终，这些知识将允许合理修改植物器官生长，这对作物性能有明显的影响。除了解决具有战略意义的基本生物学问题外，我们的工作还将在英国和巴西机构之间建立新的互利的长期科学联系。

项目成果

DELLA genes restrict inflorescence meristem function independently of plant height.

• DOI: 10.1038/s41477-017-0003-y
• 发表时间: 2017-09
• 期刊: Nature plants
• 影响因子: 18
• 作者: Serrano-Mislata A;Bencivenga S;Bush M;Schiessl K;Boden S;Sablowski R
• 通讯作者: Sablowski R

Active Control of Cell Size Generates Spatial Detail during Plant Organogenesis.

• DOI: 10.1016/j.cub.2015.10.008
• 发表时间: 2015-11-16
• 期刊: Current biology : CB
• 作者: Serrano-Mislata A;Schiessl K;Sablowski R
• 通讯作者: Sablowski R

Control of patterning, growth, and differentiation by floral organ identity genes.

通过花器官识别基因控制图案形成、生长和分化。

• DOI: 10.1093/jxb/eru514
• 发表时间: 2015
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Sablowski R

Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis.

• DOI: 10.1016/j.devcel.2016.08.013
• 发表时间: 2016-10-24
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Bencivenga, Stefano;Serrano-Mislata, Antonio;Bush, Max;Fox, Samantha;Sablowski, Robert
• 通讯作者: Sablowski, Robert

Roots of beauty.

美的根源。

• DOI: 10.1038/nrm3559
• 发表时间: 2013
• 期刊: Nature reviews. Molecular cell biology
• 作者: Sablowski R