Defining the symbiotic calcium channel of legumes

定义豆类的共生钙通道

• 批准号: BB/J008737/1
• 负责人: Giles Oldroyd
• 金额: $ 78.66万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ008737%2F1
• 关键词: Defining; symbiotic; calcium; channel; legumes

Cells have the capacity to recognise a wide array of signalling molecules and such recognition is essential to allow coordinated development in multicellular organisms. Calcium is commonly used as a secondary messenger that allows the recognition of a signal, for instance on the cell surface, to be transmitted to other regions of the cell, for instance the nucleus where gene regulation occurs. The potency of calcium as a signalling molecule and the fact that sustained calcium levels in the cytoplasm are toxic, means that calcium levels are tightly regulated. Calcium is either pumped outside the cell or into intracellular compartments such as the endoplasmic reticulum, the vacuole and vesicles. The release of calcium into the cytosol to act in signalling is the function of calcium channels that reside on the membranes of these compartments and allow the flow of calcium with its concentration gradient into the cytoplasm or nucleoplasm. Proteins that function as calcium channels have been extensively studied in animal systems and a number of channel complexes have been shown to regulate calcium. Many of the animal calcium channels are absent in plants and this has hindered the characterisation of calcium signalling in plant systems. The proteins responsible for regulation of calcium in plant cells are only just beginning to be discovered. In this proposal we will define the calcium channels responsible for calcium oscillations that occur in the symbiosis signalling pathway of legumes. This signalling pathway is responsible for the establishment of nitrogen-fixing bacterial associations as well as beneficial fungal associations that occur in legume roots. We have already identified a strong candidate for the symbiotic calcium channel and in this proposal we will further validate the role of this candidate calcium channel in symbiosis signalling, as well as define the components of the channel complex that is likely to reside on the nuclear membranes.

细胞具有识别多种信号分子的能力，这种识别对于多细胞生物的协调发育至关重要。钙通常被用作辅助信使，允许识别信号，例如在细胞表面，传递到细胞的其他区域，例如基因调控发生的细胞核。钙作为一种信号分子的效力，以及细胞质中持续的钙水平是有毒的这一事实，意味着钙水平受到严格调节。钙要么被泵入细胞外，要么被泵入细胞内腔室，如内质网、液泡和囊泡。钙离子释放到细胞质中并在信号传导中起作用，这是位于这些隔室膜上的钙离子通道的功能，它允许钙离子以其浓度梯度流入细胞质或核质。作为钙通道的蛋白质已经在动物系统中得到了广泛的研究，许多通道复合物已经被证明可以调节钙。许多动物钙通道在植物中不存在，这阻碍了植物系统中钙信号的表征。在植物细胞中负责调节钙的蛋白质才刚刚开始被发现。在本提案中，我们将定义在豆科植物共生信号通路中负责钙振荡的钙通道。该信号通路负责建立固氮细菌关联以及发生在豆科植物根部的有益真菌关联。我们已经确定了一个强候选共生钙通道，在本提案中，我们将进一步验证该候选钙通道在共生信号传导中的作用，并定义可能位于核膜上的通道复合物的成分。

项目成果

The role of DMI1 in establishing Ca (2+) oscillations in legume symbioses.

• DOI: 10.4161/psb.22894
• 发表时间: 2013-02
• 期刊: Plant signaling & behavior
• 影响因子: 2.9
• 作者: Charpentier M;Vaz Martins T;Granqvist E;Oldroyd GE;Morris RJ
• 通讯作者: Morris RJ

Nuclear Calcium Signaling in Plants

• DOI: 10.1104/pp.113.220863
• 发表时间: 2013-10-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Charpentier, Myriam;Oldroyd, Giles E. D.
• 通讯作者: Oldroyd, Giles E. D.