MECHANISM OF ACTION OF INTERFERON AGAINST HIV
MECHANISM OF ACTION OF INTERFERON AGAINST HIV
批准号:
3145312
负责人:
Bertram L. Jacobs
金额:
$13.4万
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-04-01 至 1994-03-31
关键词:
AIDS SDS polyacrylamide gel electrophoresis T lymphocyte adenosine monophosphate antiAIDS agent binding proteins double stranded RNA enzyme induction /repression enzyme mechanism fluorescence spectrometry genetic manipulation human immunodeficiency virus human subject immunologic assay /test interferons laboratory rabbit molecular cloning monocyte northern blottings pancreatic ribonuclease pharmacokinetics phosphorylation protein biosynthesis protein kinase radiotracer southern blotting tissue /cell culture virus assembly virus protein western blottings
中文摘要
干扰素和 dsRNA 是抗病毒药物,已显示出作为治疗药物的前景。
对抗艾滋病毒感染。 本研究中描述的工作
该提案将确定干扰素和 dsRNA 抑制的机制
HIV在急性感染细胞中的复制。 拟议的实验
将确定 HIV 在急性感染细胞中复制的位点
对干扰素 n 治疗敏感。实验将是
进行以确定干扰素诱导的途径是否已被
dsRNA 依赖性 eF-2alpha 参与抑制其他病毒
蛋白激酶和 2',5' 寡腺苷酸合成酶/RNase L 途径,
在 HIV 感染的细胞中被激活。 这些的潜在抑制剂
途径将在易感染 HIV 的细胞系中表达
提供有关这些途径重要性的直接信息
抑制 HIV 复制。
由于干扰素和 dsRNA 正在作为组合在患者中进行测试
与其他抗病毒药物一起治疗,基本的生物学、生化和
HIV与病毒之间相互作用的分子生物学研究
本申请中提出的干扰素系统可以提供见解
允许更好地使用干扰素和增强干扰素的药物
临床上的抗病毒活性。
英文摘要
Interferons and dsRNA are anti-virals that have shown promise as agents in
combatting infection with HIV. The work described in this research
proposal will determine the mechanism by which interferon and dsRNA inhibit
replication of HIV in acutely-infected cells. The proposed experiments
will determine the site in replication of HIV in acutely-infected cells
that is sensitive to treatment with interferon n. Experiments will be
performed to determine if the interferoninduced pathways that have been
implicated in inhibition of other viruses, the dsRNAdependent elF-2alpha
protein kinase and the 2',5' oligo-adenylate synthetase/RNase L pathway,
are activated in HIV-infected cells. Potential inhibitors of these
pathways will be expressed in cell lines susceptible to infection with HIV
to provide direct information concerning the importance of these pathways
in inhibition of HIV replication.
Since interferon and dsRNA are being tested in patients as a combination
therapy with other anti-virals, the basic biological, biochemical, and
molecular biological studies of the interactions between HIV and the
interferon system proposed in this application may provide insights to
allow better use of interferon, and agents that augment interferon's
anti-viral activity in the clinic.
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会议论文
Monkeypox virus, Interferon, and Necroptosis
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批准号:10678914
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项目类别:
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资助金额:$41.83万
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财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
Monkeypox virus, Interferon, and Necroptosis
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批准号:10241530
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项目类别:
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资助金额:$47.68万
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财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
DsRNA Characterization in Monkeypox-infected Cells
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批准号:8372121
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项目类别:
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资助金额:$38.3万
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财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
Monkeypox virus, Interferon, and Necroptosis
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批准号:10455608
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项目类别:
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资助金额:$54.12万
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财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
DsRNA Characterization in Monkeypox-infected Cells
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批准号:8653927
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项目类别:
-
资助金额:$38.3万
-
财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
Monkeypox virus, Interferon, and Necroptosis
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批准号:10052748
-
项目类别:
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资助金额:$53.83万
-
财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
DsRNA Characterization in Monkeypox-infected Cells
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批准号:8473158
-
项目类别:
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资助金额:$36.0万
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财政年份:2012
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负责人:Bertram L. Jacobs
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依托单位:
XVII International Poxvirus, Asfivirus and Indorviurs Symposium
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批准号:8006015
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项目类别:
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资助金额:$1.5万
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财政年份:2010
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负责人:Bertram L. Jacobs
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依托单位:
Disabling Vaccinia IFNr: A New Smallpox Vaccine
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批准号:7491011
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项目类别:
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资助金额:$78.44万
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财政年份:2005
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负责人:Bertram L. Jacobs
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依托单位:
Development of a post-exposure vaccine for smallpox
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批准号:6998645
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项目类别:
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资助金额:$109.74万
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财政年份:2005
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负责人:Bertram L. Jacobs
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依托单位:
Disabling Vaccinia IFNr: A New Smallpox Vaccine
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批准号:7091654
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项目类别:
-
资助金额:$41.81万
-
财政年份:2005
-
负责人:Bertram L. Jacobs
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依托单位:
Disabling Vaccinia IFNr: A New Smallpox Vaccine
-
批准号:7285053
-
项目类别:
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资助金额:$10.84万
-
财政年份:2005
-
负责人:Bertram L. Jacobs
-
依托单位:
Disabling Vaccinia IFNr: A New Smallpox Vaccine
-
批准号:7283015
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项目类别:
-
资助金额:$77.77万
-
财政年份:2005
-
负责人:Bertram L. Jacobs
-
依托单位:
Disabling Vaccinia IFNr: A New Smallpox Vaccine
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批准号:6988269
-
项目类别:
-
资助金额:$49.33万
-
财政年份:2005
-
负责人:Bertram L. Jacobs
-
依托单位:
Development of a Safer Smallpox Vaccine
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批准号:6802262
-
项目类别:
-
资助金额:$93.26万
-
财政年份:2003
-
负责人:Bertram L. Jacobs
-
依托单位:
Development of a Safer Smallpox Vaccine
-
批准号:7086393
-
项目类别:
-
资助金额:$128.55万
-
财政年份:2003
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负责人:Bertram L. Jacobs
-
依托单位:
Role of the E3L gene in poxvirus pathogenesis
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批准号:7159400
-
项目类别:
-
资助金额:$35.1万
-
财政年份:2003
-
负责人:Bertram L. Jacobs
-
依托单位:
Development of a Safer Smallpox Vaccine
-
批准号:7283126
-
项目类别:
-
资助金额:$112.4万
-
财政年份:2003
-
负责人:Bertram L. Jacobs
-
依托单位:
Role of the E3L gene in poxvirus pathogenesis
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批准号:6840547
-
项目类别:
-
资助金额:$35.33万
-
财政年份:2003
-
负责人:Bertram L. Jacobs
-
依托单位:
Role of the E3L gene in poxvirus pathogenesis
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批准号:7017107
-
项目类别:
-
资助金额:$35.31万
-
财政年份:2003
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负责人:Bertram L. Jacobs
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依托单位: