FUNCTIONAL DOMAINS OF BACILLUS THURINGIENSIS ENDOTOXIN

苏云金芽孢杆菌内毒素的功能域

• 批准号: 3143787
• 负责人: DONALD H DEAN
• 金额: $ 12.18万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3143787
• 关键词: Bacillus; Lepidoptera; bacterial genetics; bacterial proteins; bioassay; biotechnology; cell adhesion; cytogenetics; cytotoxicity; endotoxins; genetic manipulation; genetic regulation; insecticides; molecular genetics; peptide chemical synthesis; pest control; pesticide interaction; protein sequence; protein structure function; site directed mutagenesis; species difference; structural genes; tissue /cell culture

The Bacillus thuringiensis delta-endotoxin is a commercially important pesticide which has specific insecticidal activity on a number of insects. Because it is a biodegradable protein and is not toxic to vertebrates and most non-target insects, it has a low environmental impact. Numerous derivatives are available due to the existence of several genes which encode the delta-endotoxin. As a protein, it has many interesting properties making it ideal for intensive biochemical studies. It is expressed in vivo as a crystal and is developmentally expressed only during sporulation. As a protein toxin, it shares certain properties with other protein toxins, but also has unique features which need to be further clarified. Funds are requested to use genetic engineering and protein engineering techniques to localize the domains of the protein which affect insect specificity, bind to tissue culture cells, and cause cytolytic damage. Changes in the toxin amino acid sequence, which will be performed in this project, are aimed at improving the specificity and activity of the toxin toward certain insect crop pests.

苏云金芽孢杆菌δ-内毒素是商业上重要的 对许多昆虫具有特定杀虫活性的杀虫剂。 因为它是一种可生物降解的蛋白质，对脊椎动物无毒， 大多数非目标昆虫，它对环境的影响很小。许多 由于存在几种基因， 编码δ-内毒素作为一种蛋白质， 使其成为深入的生物化学研究的理想选择。是 在体内以晶体形式表达，并且仅在发育过程中表达。 孢子形成作为一种蛋白质毒素，它与其他毒素具有某些特性， 蛋白质毒素，但也有独特的功能，需要进一步 明确了申请资金用于基因工程和蛋白质 工程技术来定位蛋白质的结构域， 昆虫特异性，与组织培养细胞结合，并引起细胞溶解 损害毒素氨基酸序列的变化，这将被执行 在这个项目中，旨在提高特异性和活性， 对某些农作物害虫的毒素。