FUNCTIONAL DOMAINS OF BACILLUS THURINGIENSIS ENDOTOXIN

苏云金芽孢杆菌内毒素是一种具有重要商业价值的 对多种昆虫具有特定杀虫活性的杀虫剂。 因为它是一种可生物降解的蛋白质，对脊椎动物和 大多数非靶标昆虫，它对环境的影响很小。数不胜数 衍生品之所以可用，是因为存在几个基因 编码三角洲内毒素。作为一种蛋白质，它有许多有趣的东西 它的特性使其成为密集生物化学研究的理想选择。它是 在体内以晶体的形式表达，只有在发育过程中才表达 产孢子。作为一种蛋白质毒素，它与其他 蛋白质毒素，还具有独特的功能，还需要进一步研究 澄清了。使用基因工程和蛋白质需要资金 定位影响蛋白质结构域的工程技术 昆虫专一性，与组织培养细胞结合，并引起细胞溶解 损坏。毒素氨基酸序列的变化，这将被执行 在这个项目中，旨在提高特异性和活性的 对某些农作物害虫的毒素。

The Bacillus thuringiensis delta-endotoxin is a commercially important pesticide which has specific insecticidal activity on a number of insects. Because it is a biodegradable protein and is not toxic to vertebrates and most non-target insects, it has a low environmental impact. Numerous derivatives are available due to the existence of several genes which encode the delta-endotoxin. As a protein, it has many interesting properties making it ideal for intensive biochemical studies. It is expressed in vivo as a crystal and is developmentally expressed only during sporulation. As a protein toxin, it shares certain properties with other protein toxins, but also has unique features which need to be further clarified. Funds are requested to use genetic engineering and protein engineering techniques to localize the domains of the protein which affect insect specificity, bind to tissue culture cells, and cause cytolytic damage. Changes in the toxin amino acid sequence, which will be performed in this project, are aimed at improving the specificity and activity of the toxin toward certain insect crop pests.