PERINATAL HIV-IMMUNE FACTORS IN MOTHERS AND INFANTS

描述：（改编自申请人摘要）拟定研究 将对已从感染艾滋病毒的母亲身上采集的样本进行 和他们的后代，以及将提供的样本，从 正在对艾滋病毒阳性孕妇进行前瞻性研究。 的因素 在孕妇中评估的将包括：（1）功能性抗体 中和同源病毒株或介导ADCC，抗体 对包膜有反应性的包膜抗原抗体的合成， 外周血B细胞;和c）细胞毒性T细胞和前体。 的 这些抗体转移到他们的婴儿将在选定的检查 母婴配对 由于ADCC可能是一个主要的具体 在胎儿/新生儿中消除早期感染细胞的机制 阶段，脐带血淋巴细胞，单核细胞和 将确定多形细胞介导该功能。 新生儿免疫细胞的差异可能是感染的目标， 包括单核细胞，记忆和幼稚CD 4+淋巴细胞，将被 研究了对HIV感染的易感性、潜伏期和复制， 垂直传播和非传播的病毒株。 近期 发现记忆/幼稚T细胞数量的变化， 在>2个月大的HIV感染婴儿中， 表明需要定量分析高危婴儿的此类细胞 出生后早期。 将检查这些免疫学研究的结果 关于从临床流行病学、病毒学和 在同一队列中进行早期诊断研究。 分析 与母婴传播或不传播的可能相关性 艾滋病毒应有助于确定关键的免疫因素， 定义传输的定时。

DESCRIPTION: (Adapted from the applicant's abstract) The proposed studies will be conducted on specimens already collected from HIV infected mothers and their offspring, as well as on samples that will be available, from ongoing prospective studies of HIV + pregnant women. Factors to be evaluated in the pregnant women will include: (1) functional antibodies which neutralize homologous strains of virus or mediate ADCC, antibodies reactive to envelope synthesis of antibodies to the envelope antigens by peripheral blood B cells; and c) cytotoxic T cells and precursors. The transfer of these antibodies to their infant will be examined in selected maternal/infant pairs. Since ADCC is likely to be a major specific mechanism in the fetus/neonate to eliminate infected cells during the early stage, the ability of cord blood lymphocytes, monocytes and polymorphonuclear cells to mediate this function will be ascertained. Differences in neonatal immune cells likely to be targets of infection, including monocytes, and memory and naive CD4 + lymphocytes, will be studied for susceptibility to HIV infection, latency and replication, with both vertically-transmitted and non-transmitted virus strains. The recent findings of variations in the number of memory/naive T-cells and of activated mononuclear cells in HIV infected infants >2 months of age also indicate the need of quantitative analysis of such cells in at-risk babies early after birth. Results of these immunological studies will be examined in relation to data collected from clinico-epidemiological, virological and early diagnostic studies being conducted in the same cohort. Analyses of possible correlation to maternal-infant transmission or nontransmission of HIV should help to identify critical immune factors and to assist in defining the timing of transmission.