Tissue specific aquaporin expression for seedling water stress resistance

组织特异性水通道蛋白表达用于幼苗抗水胁迫

• 批准号: BB/J017582/1
• 负责人: Lorenzo Frigerio
• 金额: $ 55.94万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FJ017582%2F1
• 关键词: Tissue; specific; aquaporin; expression; seedling

This plant science project combines cell biology and plant physiology to understand how seeds take up water during germination and post-germination seedling growth and how we can control this process to develop crops that can germinate and establish in water stress conditions. Rapid plant tissue expansion after seed germination is crucial for crop establishment and plant uniformity and it directly impacts on quality and yield at the end of the crop cycle. For these reasons early vigour is an important target for crop improvement. Most cells (including seed cells) take up water through protein water channels called aquaporins (AQP). While a lot is known about AQP structure and function in plant roots and leaves, very little is known about their role in seed germination and post-germination seedling growth. Our project directly addresses this question. AQP exist on different cellular membranes and water intake is usually controlled by AQP on the outer cell membrane (the plasma membrane). Remarkably, we have found that in seeds the AQP that are normally present on the plasma membrane are NOT produced until AFTER the seed has germinated. So what is controlling water entry during germination? We have discovered that AQP that normally reside on the membrane of the vacuole (the tonoplast) are also found at the plasma membrane during seed maturation and germination. Therefore it seems that seeds have a unique way of regulating water intake by sending tonoplast AQP to the plasma membrane.We have also discovered that plants modified to produce very high amounts of seed AQP are able to germinate and grow in conditions of water stress, performing significantly better than wild-type plants. Therefore we hypothesise that seed AQP are major controllers of water flow in seeds and early seedling growth that can be used to generate plants that cope better with drought conditions.Our project aims at understanding the role of AQP by studying the way the tonoplast AQP are targeted to the plasma membrane. We also propose to manipulate both the amount and type of AQP present on the seed cells' plasma membrane and correlate it with the ability of seeds to germinate and the vigour of post-germination seedling growth using a range of water stress conditions. We will perform the basic experiments in the model plant Arabidopsis but, in parallel we will also perform pilot experiments in Brassica oleracea, a crop for which co-ordinated germination and early vigour can have a direct, major impact on final yield. By the end of the project we aim to have improved understanding of the regulation of AQP during germination and early sedling growth and to have established strategies to manipulate AQP in order to maximise early vigour and water stress resistance.

这个植物科学项目结合了细胞生物学和植物生理学，以了解种子在发芽和发芽后幼苗生长过程中如何吸收水分，以及我们如何控制这一过程，以开发出能够在水分胁迫条件下发芽和生长的作物。种子萌发后植物组织的快速扩展对于作物的建立和植株的整齐度至关重要，并且在作物周期结束时直接影响质量和产量。由于这些原因，早期活力是作物改良的重要目标。大多数细胞（包括种子细胞）通过称为水通道蛋白（AQP）的蛋白质水通道摄取水分。虽然对植物根和叶中AQP的结构和功能了解很多，但对它们在种子萌发和萌发后幼苗生长中的作用知之甚少。我们的项目直接解决了这个问题。水通道蛋白存在于不同的细胞膜上，水的摄入通常由细胞外膜（质膜）上的水通道蛋白控制。值得注意的是，我们发现在种子中，通常存在于质膜上的AQP直到种子发芽后才产生。那么，是什么控制了发芽过程中的水分进入呢？我们已经发现，AQP，通常居住在液泡膜（液泡膜）也发现在质膜种子成熟和萌发过程中。因此，种子似乎有一种独特的方式来调节水分的摄入量，通过发送液泡AQP到质膜。我们还发现，植物改造，以产生非常高的种子AQP量能够发芽和生长在水分胁迫条件下，表现明显优于野生型植物。因此，我们假设种子水通道蛋白是种子中水流动和早期幼苗生长的主要控制者，可以用来产生更好地科普干旱条件的植物。我们的项目旨在通过研究液泡膜水通道蛋白靶向质膜的方式来了解水通道蛋白的作用。我们还建议操纵种子细胞质膜上存在的AQP的量和类型，并将其与种子发芽的能力和使用一系列水分胁迫条件的发芽后幼苗生长的活力相关联。我们将在模式植物拟南芥中进行基本实验，但同时我们也将在甘蓝中进行中试实验，甘蓝是一种协调发芽和早期活力对最终产量有直接重大影响的作物。在项目结束时，我们的目标是提高对发芽和早期定居生长过程中AQP调节的理解，并建立策略来操纵AQP，以最大限度地提高早期活力和水分胁迫抗性。

项目成果

Arabidopsis Lunapark proteins are involved in ER cisternae formation.

• DOI: 10.1111/nph.15228
• 发表时间: 2018-08
• 期刊: The New phytologist
• 作者: Kriechbaumer V;Breeze E;Pain C;Tolmie F;Frigerio L;Hawes C
• 通讯作者: Hawes C

Reprogramming cells to study vacuolar development.

• DOI: 10.3389/fpls.2013.00493
• 发表时间: 2013
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Feeney M;Frigerio L;Kohalmi SE;Cui Y;Menassa R
• 通讯作者: Menassa R

Arabidopsis Lunapark proteins are involved in ER cisternae formation

• DOI: 10.1101/256743
• 发表时间: 2018-01
• 期刊: The New Phytologist
• 作者: V. Kriechbaumer;E. Breeze;Charlotte Pain;Frances Tolmie;L. Frigerio;C. Hawes