THE GLUCOREGULATORY PEPTIDE HORMONES

在接下来的一年里，我们希望对人类的D细胞功能进行表征。之后 经过一年的努力，我们还没有开发出一种实用的放射免疫分析方法 未提取的人血浆中的生长抑素，就像我们以前在狗身上做的那样。我们 已经定义了这种化验的某些技术障碍，而且似乎 内源性生长抑素的快速降解与蛋白水解物的结合 破坏生长抑素示踪剂，构成主要障碍。我们计划 测试四种提取或分离工艺的有效性 绕过全血浆检测的问题。其中包括凝胶过滤， 丙酮提取、免疫亲和层析和离子交换 层析法。到目前为止，观察到的最佳恢复是 免疫亲和层析法。我们现在的努力旨在使 该技术适用于生理学和生物制品所需的大容量分析工作 人类的病理生理学研究。如果这一点实现，我们计划 研究正常人对进食和其他动作的生长抑素反应 受试者和肥胖受试者以及各种形式的糖尿病患者， 胃肠道疾病和脑下垂体疾病。我们对这个问题的兴趣 可能是因为生长抑素作为营养的调节剂 肠腔向内环境的移动，可能在 广泛的人类疾病。 与Raskin博士和Pietri博士合作进行的临床研究 I型糖尿病患者肌肉毛细血管基底区代谢近正常化 膜厚度、肾功能、运动神经传导速度、高密度脂蛋白-2和 低密度脂蛋白水平，视网膜荧光血管造影术将持续到第二个月 年。

During the coming year we hope to characterize D-cell function in man. After one year of effort, we have yet to develop a practical radioimmunoassay for somatostatin in unextracted human plasma, as we had done previously in dogs. We have defined certain of the technical obstacles to such an assay and it appears that rapid degradation of endogenous somatostatin, coupled with proteolytic damage to the somatostatin tracer, constitute the main obstacles. We plan to test the efficacy of four extraction or isolation technics by which to circumvent the problems of whole plasma assay. These include gel filtration, acetone extraction, immunoaffinity chromatography and ion exchange chromatography. The best recoveries have thus far been observed with immunoaffinity chromatography methods. Our efforts now are designed to make such technics practical for large volume assay work required for physiologic and pathophysiologic studies in man. If and when this is accomplished, we plan to study the somatostatin response to meals and to other maneuvers in normal subjects and in obese subjects as well as in the various forms of diabetes, in gastrointestinal disorders and pituitary disease. Our interest in this problem is prompted by the possibility that somastatin, as the regulator of nutrient movement of the gut lumen to the internal environment, may be important in a broad spectrum of human disorders. In collaboration with Drs. Raskin and Pietri a clinical study of the efficacy of metabolic near-normalization of Type I diabetics upon muscle capillary basement membrane thickness, renal function, motor nerve conduction velocity, HDL-2 and LDL levels, and retinal fluorescin angiography will continue into its second year.