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PRIMARY AND SECONDARY BINDING SITES OF THE TRANSFERRINS

PRIMARY AND SECONDARY BINDING SITES OF THE TRANSFERRINS
转铁蛋白的主要和次要结合位点
批准号:
3151090
负责人:
GEORGE W BATES
金额:
$21.97万
依托单位国家:
美国
项目类别:
财政年份:
1977
资助国家:
美国
项目状态:
已结题
起止时间:
1977-12-01 至 1987-11-30

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项目成果

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中文摘要
翻译
研究计划可分为五个部分。
英文摘要
The Research Plan can be divided into five sections. 1. Secondary metal binding sites of sero- and lactotransferrin. Evidence from amino acid sequence comparisons, EPR, kinetics and spectrophotometry reveal the presence of secondary metal binding sites. I wish to ascertain their stoichiometry, relative affinities for different metals, metal exchange reactivities and their effect on the primary metal/anion sites. 2. Iron exchange reactivities of lactotransferrin. Little is known about the reactions in which lactotransferrin attacks, sequesters and releases iron. This information will shed light on possible physiological roles of lactotransferrin and provide a valuable comparison with the data on serotransferrin. 3. Environmental and chemical modification and the iron exchange reactivities of the transferrins. Denaturants, pH, chemical modification, temperature and salts have a differential effect on the sites in the transferrins' two lobes. I want to know how the modification alters the attack, sequestration and iron release properties. Of special interest will be insight into the conformational change that results in the labilization of the iron and anion binding stress. 4. Control of attack, sequestration and release of iron by the anion binding site. The anion binding site controls the affinity of the protein for iron and other metals. This phenomenon can be explored via the use of carbonate substitutes in studies of metal ion attack, sequestration, oxidation, reduction and release. 5. Exchange of metals other than iron by the transferrins. The transferrins offer an excellent model system for examining protein metal ion exchange for Cu, Mn, Co, Cr, Ni and others. I want to discover the rules that govern exchange of metal ions and to learn about the mechanisms that direct metals to specific sites. 6. Metal ion exchange between the transferrins and other proteins. How are metal ions passed from one protein to another? Is it always via mediators such as chelators and redox reagents? or via a modification force such as acidification? Do protein-metal-protein complexes form? Ceruloplasmin, phosvitin, the sero-, ovo- and lactotransferrins, serum albumin, carboxypeptidase A, ferritin will be subjects of study. 7. Lactotransferrin in milk and serotransferrin in serum. Too little attention is paid to the metal ion exchange reactivities in the natural environment. I wish to extend much of the work above to the physiological media of interest.
期刊论文(5)
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会议论文
The effect of chemical agents, beverages, and spinach on the in vitro solubilization of iron from cooked pinto beans.
化学制剂、饮料和菠菜对煮熟的斑豆中铁的体外溶解的影响。
DOI: 10.1093/ajcn/34.7.1392
发表时间: 1981
期刊: The American journal of clinical nutrition
影响因子: --
作者: [Kojima,N, Wallace,D, Bates,GW]
通讯作者: Bates,GW
Failure of metallothionein to bind iron or act as an iron mobilizing agent.
金属硫蛋白未能结合铁或充当铁动员剂。
DOI: 10.1016/0304-4165(82)90278-1
发表时间: 1982
期刊: Biochimica et biophysica acta
影响因子: --
作者: [Kojima,N, Young,CR, Bates,GW]
通讯作者: Bates,GW
The formation of Fe3+-transferrin-CO3(2-) via the binding and oxidation of Fe2+.
通过 Fe2 的结合和氧化形成 Fe3 -转铁蛋白-CO3(2-)。
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者: [Kojima,N, Bates,GW]
通讯作者: Bates,GW
DOI: 10.1016/s0021-9258(18)34416-8
发表时间: 1982-07
期刊: The Journal of biological chemistry
影响因子: --
作者: [R. Cowart;N. Kojima;G. Bates]
通讯作者: R. Cowart;N. Kojima;G. Bates
A CHEMICAL REACTIVITY APPROACH TO IRON BIOAVAILABILITY
PRIMARY AND SECONDARY BINDING SITES OF THE TRANSFERRINS
PRIMARY AND SECONDARY BINDING SITES OF THE TRANSFERRINS
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