CHEMISTRY OF MUSCLE PROTEINS
CHEMISTRY OF MUSCLE PROTEINS
批准号:
3151057
负责人:
SUSAN LOWEY
金额:
$18.38万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1976
资助国家:
美国
项目状态:
已结题
起止时间:
1976-01-01 至 1985-12-31
关键词:
X ray crystallography calcium chemical binding chemical fingerprinting chemical group chemical structure function crystallization electron microscopy embryo /fetus fluorescent dye /probe immunochemistry isozymes monoclonal antibody muscle contraction muscle relaxation myofibrils myogenesis nucleotides phosphorylation saltwater environment smooth muscle
中文摘要
我们研究的一个主要目的是发现低分子量的作用
英文摘要
A major aim in our research is to discover the role of the low molecular weight
subunits in myosin. After nearly a decade of effort by several laboratories,
their function in vertebrate skeletal muscle remains obscure. One assumes that
they are probably involved in the basic actomyosin interaction, but little firm
evidence is available to support this hypothesis. We will pursue two main
directions to advance this problem: (1) We will attempt to localize the two
classes of light chains in the myosin head by introducing specific markers, such
as fluorescent probes or monoclonal antibodies into defined sites on the light
and heavy chain subunits. Distances between fluorophores will be measured by
Forster energy transfer. Antibody-myosin complexes will be viewed by electron
microscopy of shadow-cast preparations. (2) We will compare the functional and
structural properties of several isoenzymes of myosin, among them fast and slow
myosins, embryonic myosin from several stages of development, and synthetic
hybrids formed by combining the subunits of slow and fast myosins. Techniques
will include kinetic analysis, two-dimensional peptide mapping, and
immunological identification of common sequences. By studying these myosin
polymorphs both in the monomeric and filamentous state, we hope to gain
additional insights into how the myosin molecule works, and why muscle is
composed of such a mosaic of different fiber types.
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DOI:
10.1016/0022-2836(81)90510-6
发表时间:
1981
期刊:
Journal of molecular biology
影响因子:
5.6
作者:
[Silberstein,L, Lowey,S]
通讯作者:
Lowey,S
Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.
平滑肌肌球蛋白的构象状态。
DOI:
--
发表时间:
1984
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Trybus,KM, Lowey,S]
通讯作者:
Lowey,S
Mechanism of smooth muscle myosin phosphorylation.
平滑肌肌球蛋白磷酸化机制。
DOI:
--
发表时间:
1985
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Trybus,KM, Lowey,S]
通讯作者:
Lowey,S
Crystallization of myosin subfragment 1.
肌球蛋白亚片段1的结晶。
DOI:
10.1073/pnas.81.14.4378
发表时间:
1984
期刊:
Proceedings of the National Academy of Sciences of the United States of America
影响因子:
11.1
作者:
[Rayment,I, Winkelmann,DA]
通讯作者:
Winkelmann,DA
DOI:
--
发表时间:
1985
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
[Waller,GS, Lowey,S]
通讯作者:
Lowey,S
共 14 条
A TG model for functional effects of FHC mutations in b-cardiac myosin
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批准号:8383309
-
项目类别:
-
资助金额:$24.2万
-
财政年份:2012
-
负责人:SUSAN LOWEY
-
依托单位:
A TG model for functional effects of FHC mutations in b-cardiac myosin
-
批准号:8509020
-
项目类别:
-
资助金额:$18.15万
-
财政年份:2012
-
负责人:SUSAN LOWEY
-
依托单位:
Specification of actomyosin function in the cell
-
批准号:8996176
-
项目类别:
-
资助金额:$29.74万
-
财政年份:2012
-
负责人:SUSAN LOWEY
-
依托单位:
MYOSIN DOMAIN INTERACTIONS DURING THE CONTRACTILE CYCLE
-
批准号:7455162
-
项目类别:
-
资助金额:$37.36万
-
财政年份:2007
-
负责人:SUSAN LOWEY
-
依托单位:
MYOSIN DOMAIN INTERACTIONS DURING THE CONTRACTILE CYCLE
-
批准号:7258277
-
项目类别:
-
资助金额:$38.29万
-
财政年份:2007
-
负责人:SUSAN LOWEY
-
依托单位:
MYOSIN DOMAIN INTERACTIONS DURING THE CONTRACTILE CYCLE
-
批准号:7648218
-
项目类别:
-
资助金额:$38.48万
-
财政年份:2007
-
负责人:SUSAN LOWEY
-
依托单位:
MYOSIN DOMAIN INTERACTIONS DURING THE CONTRACTILE CYCLE
-
批准号:8097416
-
项目类别:
-
资助金额:$38.85万
-
财政年份:2007
-
负责人:SUSAN LOWEY
-
依托单位:
MYOSIN DOMAIN INTERACTIONS DURING THE CONTRACTILE CYCLE
-
批准号:7874572
-
项目类别:
-
资助金额:$39.24万
-
财政年份:2007
-
负责人:SUSAN LOWEY
-
依托单位:
OPTIMA XL-A ANALYTICAL ULTRACENTRIFUGE
-
批准号:2285855
-
项目类别:
-
资助金额:$15.5万
-
财政年份:1994
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:2078370
-
项目类别:
-
资助金额:$39.81万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:6171453
-
项目类别:
-
资助金额:$43.84万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:2732810
-
项目类别:
-
资助金额:$40.54万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481448
-
项目类别:
-
资助金额:$32.06万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481447
-
项目类别:
-
资助金额:$26.33万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481445
-
项目类别:
-
资助金额:$23.66万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:2078368
-
项目类别:
-
资助金额:$34.87万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481444
-
项目类别:
-
资助金额:$23.05万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:6029919
-
项目类别:
-
资助金额:$42.16万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481449
-
项目类别:
-
资助金额:$31.06万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
CHEMISTRY OF MUSCLE PROTEINS
-
批准号:3481442
-
项目类别:
-
资助金额:$22.41万
-
财政年份:1976
-
负责人:SUSAN LOWEY
-
依托单位:
国内基金
海外基金
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资助金额:52万元
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批准年份:2022
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负责人:张明明
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依托单位:
miR-30调控Calcium/Calcineurin通路在慢性肾脏病心肌保护中的作用
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批准号:81670699
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项目类别:面上项目
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资助金额:58.0万元
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批准年份:2016
-
负责人:郑春霞
-
依托单位:
水稻OsCAS(Calcium-sensing Receptor)基因的功能分析
-
批准号:30900771
-
项目类别:青年科学基金项目
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资助金额:20.0万元
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批准年份:2009
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负责人:赵昕
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依托单位: