STRUCTURE OF CONTROL ENZYMES IN GLUCOSE METABOLISM

葡萄糖代谢中控制酶的结构

• 批准号: 3151646
• 负责人: ROBERT G KEMP
• 金额: $ 31.51万
• 依托单位: ROSALIND FRANKLIN UNIV OF MEDICINE & SCI
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151646
• 关键词: 6 phosphofructokinase; Bacillus stearothermophilus; Escherichia coli; Saccharomyces; adenine nucleotides; allosteric site; chemical binding; chemical group; crosslink; enzyme structure; fructose biphosphatase; gluconeogenesis; glucose metabolism; liver metabolism; phosphorylation; striated muscles

The project continues our structural studies of the two key regulatory enzymes of carbohydrate metabolism, phosphofructokinase (PFK) and fructose 1,6-bisphosphatase (FbPase). The research, which involves a major collaborative effort of three laboratories, includes the following: (1) The primary sequence of rabbit muscle PFK, which is almost complete, will be finished and predictions concerning three dimensional organization will be tested by cross-linking experiments. (2) Sequences around functional residues of muscle PFK will be determined by covalent modification techniques. (3) Predictions concerning the evolution of allosteric sites of PFK will be evaluated by functional site identification and partial sequence analysis of E. coli and yeast (Saccharomyces cerevisiae) PFK. (4) Functional site identification and sequence analysis of E. coli FbPase, and an analysis of the phosphorylation site(s) of Saccharomyces cerevisiae FbPase will be compared to data obtained with mammalian FbPases. (5) The sequence of skeletal muscle FbPase, an enzyme with increased sensitivity to AMP inhibition, will be determined and compared to the kidney and liver enzyme. These studies provide structural correlates to our knowledge of allosteric interactions and is of interest to our overall understanding of enzyme regulatory mechanisms.

该项目继续我们对这两个关键调节的结构研究 碳水化合物代谢，磷酸果糖激酶（PFK）和果糖的酶 1,6-双磷酸酶（FBPase）。 这项研究涉及主要 三个实验室的协作努力包括以下内容： （1）几乎完整的兔肌肉PFK的主要序列 将完成并有关三维组织的预测 将通过交联实验测试。 （2）将确定肌肉PFK功能残基周围的序列 通过共价修改技术。 （3）关于PFK变构位点演变的预测将是 通过功能部位识别和部分序列分析评估 大肠杆菌和酵母（酿酒酵母）的PFK。 （4）大肠杆菌FBPase的功能位点识别和序列分析， 以及酿酒酵母的磷酸化位点的分析 FBPase将与哺乳动物FBPase获得的数据进行比较。 （5）骨骼肌FBPase的序列，一种酶增加 对AMP抑制的敏感性将确定并与 肾脏和肝酶。 这些研究提供了与我们对变构知识的了解 相互作用，我们对酶的整体理解很感兴趣 监管机制。

项目成果

Amino acid sequence similarity between malate dehydrogenases (NAD) and pea chloroplast malate dehydrogenase (NADP).

苹果酸脱氢酶（NAD）和豌豆叶绿体苹果酸脱氢酶（NADP）之间的氨基酸序列相似性。

• DOI: 10.1111/j.1432-1033.1987.tb13466.x
• 发表时间: 1987
• 期刊: European journal of biochemistry
• 作者: Fickenscher,K;Scheibe,R;Marcus,F
• 通讯作者: Marcus,F

Amino acid sequence similarity between spinach chloroplast and mammalian gluconeogenic fructose-1,6-bisphosphatase.

菠菜叶绿体和哺乳动物糖异生果糖-1,6-双磷酸酶之间的氨基酸序列相似性。

• DOI: 10.1016/0006-291x(85)90937-4
• 发表时间: 1985
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Harrsch,PB;Kim,Y;Fox,JL;Marcus,F
• 通讯作者: Marcus,F

Isolation and sequence determination of an active site peptide of rabbit muscle pyruvate kinase.

兔肌肉丙酮酸激酶活性位点肽的分离和序列测定。

• DOI: 10.1016/0003-9861(87)90645-x
• 发表时间: 1987
• 期刊: Archives of biochemistry and biophysics
• 影响因子: 3.9
• 作者: Bezares,G;Eyzaguirre,J;Hinrichs,MV;Heinrikson,RL;Reardon,I;Kemp,RG;Latshaw,SP;Bazaes,S
• 通讯作者: Bazaes,S