PROTEIN SEQUENCE EQUIPMENT

蛋白质测序设备

• 批准号: 3519156
• 负责人: ROBERT G KEMP
• 金额: $ 20.58万
• 依托单位: ROSALIND FRANKLIN UNIV OF MEDICINE & SCI
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1985-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3519156
• 关键词: aminoacid analyzer; enzyme structure; high performance liquid chromatography

The project requests three instruments as part of a peptide and protein structure determination laboratory: an amino acid analyzer, a sequencer, and a high performance liquid chromatograph. These will be used in projects to study covalent structures of phosphofructokinase, fructose bisphosphatase, the protein core of heparan sulfate, the proteins of mucus production in the hagfish and the major polypeptide antigens on the surface of a parasite. the equipment will also be used to monitor peptide metabolism in kidney tubules, to determine the composition and homogeneity of muramyl peptides of physiological significance, and to determine the N-terminal sequence of mitochondrial gene products. It is anticipated that the laboratory will provide an impetus for other studies and as a core facility that will aid in the future recruitment of research faculty.

该项目要求三种仪器作为肽和蛋白质的一部分 结构测定实验室：氨基酸分析仪，测序仪， 和高性能液相色谱。 这些将用于 研究磷酸果酶，果糖的共价结构的项目 双磷酸酶，硫酸乙酰肝素的蛋白质核心，粘液的蛋白质 在hag鱼和表面的主要多肽抗原中产生 寄生虫。 该设备还将用于监测肽 肾小管中的代谢，以确定成分和同质性 具有生理意义的穆拉米基肽，并确定 线粒体基因产物的N末端序列。 预计 实验室将为其他研究提供动力，并作为核心 将有助于未来招募研究教师的设施。