INSULIN BINDING SITES AND INSULIN ACTION

拟议的研究涉及表征黄曲霉毒素的物理化学性质 正常和胰岛素抵抗状态下的胰岛素受体。尝试次数将是 用于溶解脂肪细胞和肝血浆中的胰岛素受体 胰岛素存在或不存在时的膜都是共价交联的 到感受器。将使用聚丙烯酰胺凝胶电泳法测定 如果受体在胰岛素抵抗的发展过程中发生了变化 各州。还计划开展其他工作，以进一步研究 植物显著提高了受体的胰岛素结合亲和力 凝集素、小麦胚芽凝集素。这些研究将采用交联法 活性脂肪细胞研究中的试剂，以确定是否侧向迁移率 需要受体或其他膜成分才能获得增加的 受体亲和力和胰岛素敏感性伴随着治疗 凝集素。

The proposed studies involve characterization of the physico-chemical nature of the insulin receptor in normal and insulin-resistant states. Attempts will be made to solubilize the insulin receptor from adipocyte and liver plasma membranes either in the presence or absence of insulin covalently cross-linked to the receptor. Polyacrylamide gel electrophoresis will be used to determine if the receptor has been altered during the development of the insulin resistant states. Other work is planned to further examine the mechanism by which the insulin-binding affinity of the receptor is markedly increased by the plant lectin, wheat germ agglutinin. These studies will employ the cross-linking reagent in studies with viable fat cells to determine if lateral mobility of the receptor or of other membrane constituents is required to obtain the increase in receptor affinity and insulin-sensitivity that accompanies treatment with the lectin.