REGULATORY T AND B CELL CIRCUITS IN TRANSPLANTATION

我们计划利用小鼠单克隆抗体在实验犬， 体外和体内移植模型。 在1和2混合淋巴细胞 (MLC)、混合淋巴细胞胰岛（MLIC）和混合淋巴细胞肾（MLKC） 我们将测量单克隆抗体对犬的反应， T淋巴细胞亚群和B细胞Ia抗原。 我们还会测试单克隆抗体 针对致敏T细胞的独特型决定簇（抗特异性 主要组织相容性复合体差异和 胰岛和肾组织特异性分化抗原 细胞）。 在这样做的过程中，我们将研究来源于细胞的体外活性。 从排斥的肾脏和自由引流的胰腺血管段 同时移植的抗组织特异性与 同种免疫反应性 提高抗独特型抗体的几种策略 单克隆抗体被描绘，以及在体内实验中，他们 将用于（与抗T细胞亚群和抗Ia试剂相比） 测试对胰腺和肾脏移植的影响 因为 反复快速发展的免疫消除，脓毒症抗独特型 免疫力，我们设计了实验来诱导无反应状态。 单克隆抗体灌注抗犬Ia病毒的器官移植 描述了抗原以及一个计划， 使用抗腺泡细胞试剂检测移植物中的分泌。

We plan to utilize mouse monoclonal antibodies in experimental canine in vitro and in vivo transplantation models. In 1 and 2 mixed lymphocyte (MLC), mixed lymphocyte islet (MLIC), and mixed lymphocyte kidney (MLKC) reactions we will measure the effects of monoclonals raised against canine T lymphocyte subsets and B cell Ia antigens. We will also test monoclonals raised against idiotypic determinants of primed T cells (anti-specific receptor sites for major histocompatibility complex disparities and for tissue specific differentiation antigens on pancreatic islet and kidney cells). In so doing we will study the in vitro activity of cells derived from rejecting kidneys and free draining pancreatic vascularized segments transplanted simultaneously with respect to anti-tissue specific vs. alloimmune reactivity. Several strategies in raising anti-idiotypic monoclonals are delineated, as well as in vivo experiments in which they will be used (compared with anti-T cell subset and anti-Ia reagents) to test effects on pancreatic and kidney transplantation. Because of the repeated rapid development of immune elimination, putatively anti-idiotypic immunity, we have designed experiments to induce an unresponsive state. Transplantation of organs perfused with monoclonals against canine Ia antigens are described as well as a plan to inactivate pancreatic acinar secretion in transplants using an anti-acinar cell reagent.