REGULATORY T AND B CELL CIRCUITS IN TRANSPLANTATION

移植中的 T 细胞和 B 细胞调节回路

• 批准号: 3151479
• 负责人: Joshua Miller
• 金额: $ 18.84万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151479
• 关键词: B lymphocyte; T lymphocyte; affinity chromatography; antiantibody; antiidiotype antibody; autoantibody; clone cells; density gradient ultracentrifugation; flow cytometry; gel electrophoresis; histochemistry /cytochemistry; histocompatibility antigens; histopathology; homologous transplantation; human subject; hybridomas; immune adherence reaction; immunochemistry; immunogenetics; immunosuppression; kidney transplantation; leukocyte activation /transformation; mixed lymphocyte reaction test; monoclonal antibody; pancreas transplantation; pancreatic islet transplantation; radiotracer; suppressor T lymphocyte; transplantation

We plan to utilize mouse monoclonal antibodies in experimental canine in vitro and in vivo transplantation models. In 1 and 2 mixed lymphocyte (MLC), mixed lymphocyte islet (MLIC), and mixed lymphocyte kidney (MLKC) reactions we will measure the effects of monoclonals raised against canine T lymphocyte subsets and B cell Ia antigens. We will also test monoclonals raised against idiotypic determinants of primed T cells (anti-specific receptor sites for major histocompatibility complex disparities and for tissue specific differentiation antigens on pancreatic islet and kidney cells). In so doing we will study the in vitro activity of cells derived from rejecting kidneys and free draining pancreatic vascularized segments transplanted simultaneously with respect to anti-tissue specific vs. alloimmune reactivity. Several strategies in raising anti-idiotypic monoclonals are delineated, as well as in vivo experiments in which they will be used (compared with anti-T cell subset and anti-Ia reagents) to test effects on pancreatic and kidney transplantation. Because of the repeated rapid development of immune elimination, putatively anti-idiotypic immunity, we have designed experiments to induce an unresponsive state. Transplantation of organs perfused with monoclonals against canine Ia antigens are described as well as a plan to inactivate pancreatic acinar secretion in transplants using an anti-acinar cell reagent.

我们计划将小鼠单克隆抗体应用于实验性犬体内

项目成果

Toward the definition of nominal tissue-associated kidney tubular cell-surface epitope(s) which cause autologous and allogeneic T cell immune reactivity.

定义引起自体和同种异体 T 细胞免疫反应的名义组织相关肾小管细胞表面表位。

• 发表时间: 1989
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Esquenazi,V;Fuller,L;Carreno,M;Roth,D;Milgrom,M;Burke,G;Olson,L;Miller,J
• 通讯作者: Miller,J

In vivo and in vitro induction of class II molecules on canine renal cells and their effect on the mixed lymphocyte kidney cell culture.

II类分子对犬肾细胞的体内和体外诱导及其对混合淋巴细胞肾细胞培养物的影响。

• DOI: 10.1097/00007890-198711000-00017
• 发表时间: 1987
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Esquenazi,V;Fuller,L;Pardo,V;Roth,D;Milgrom,M;Miller,J
• 通讯作者: Miller,J

Comparison of two monoclonal antibodies affecting canine transplantation reactions.

两种单克隆抗体影响犬移植反应的比较。

• 发表时间: 1987
• 期刊: Transplantation proceedings
• 影响因子: 0.9
• 作者: Carreno,M;Esquenazi,V;Olson,L;Fuller,L;Alejandro,R;Milgrom,M;Miller,J
• 通讯作者: Miller,J

Factors affecting the autologous mixed lymphocyte reaction in kidney transplantation.

肾移植中自体混合淋巴细胞反应的影响因素

• DOI: 10.1172/jci110883
• 发表时间: 1983
• 期刊: The Journal of clinical investigation
• 作者: Fuller,L;Flaa,C;Jaffe,D;Strauss,J;Kyriakides,GK;Miller,J
• 通讯作者: Miller,J

The biologic significance of the mixed lymphocyte kidney culture in humans.

人类混合淋巴细胞肾培养的生物学意义。

• DOI: 10.1097/00007890-198510000-00007
• 发表时间: 1985
• 期刊: Transplantation
• 影响因子: 6.2
• 作者: Roth,D;Fuller,L;Esquenazi,V;Kyriakides,GK;Pardo,V;Miller,J
• 通讯作者: Miller,J