Factors affecting the autologous mixed lymphocyte reaction in kidney transplantation.
Factors affecting the autologous mixed lymphocyte reaction in kidney transplantation.
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肾移植中自体混合淋巴细胞反应的影响因素
DOI：
10.1172/jci110883
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Miller,J
中科院分区：
文献类型：
--
作者：
Fuller,L;Flaa,C;Jaffe,D;Strauss,J;Kyriakides,GK;Miller,J
在长期适应良好的肾移植受者中，我们发现T辅助细胞（TH）：T抑制细胞/细胞毒性（TS/C）亚群比例与自体混合淋巴细胞反应（AMLR）中T细胞的存在密切相关。在21例T细胞E花环率在53 ~ 86%之间，TH：TS/C比值在0.15 ~ 2.10之间的受者中，比值大于0.8与AMLR反应性相关（13/13），比值小于0.8与AMLR无反应性相关（7/7）。相比之下，同种异体MLR与TH：TS/C比值或术前或术后AMLR无明显相关性。结果发现，23名正常人中有22名的AMLR被从外周血淋巴细胞获得的自体T细胞显著抑制，暴露于3，000 rad（Tx）并作为第三组分添加到培养物中。相比之下，13个肾移植受体中的13个未能表现出这种Tx AMLR抑制细胞群。“自然发生的”T抑制细胞，通过亲和柱层析程序分成X-照射的TH和TS/C亚群，抑制AMLR到与未分离的Tx细胞相同的程度。在5个HLA相同的供体-受体对中的4个中进行的细胞交换研究中，（正常）供体的Tx细胞抑制受体AMLR（免疫抑制），但受体Tx细胞未能抑制供体AMLR。最后，测试在AMLR和同种异体MLR中致敏10天的T细胞的AMLR或同种异体MLR抑制活性。同种异体MLR引发的X射线照射的细胞，抑制AMLR和同种异体MLR，而AMLR X射线照射引发的细胞抑制两种反应。在正常外周血中发现的Tx AMLR抑制剂似乎是对生物或药理学免疫抑制剂的作用高度敏感的细胞。
In long-term well adapted kidney transplant recipients we have found a close correlation between the T helper (TH):T suppressor/cytotoxic (TS/C) subset ratios and the presence of T cells that respond in the autologous mixed lymphocyte reaction (AMLR). In 21 recipients with T cell E rosette levels ranging between 53 and 86% and TH:TS/C ratios between 0.15 to 2.10, ratios of greater than 0.8 correlated with AMLR responses (13/13), and ratios of less than 0.8 with AMLR nonreactivity (7/7). By contrast, the allogeneic MLR showed no apparent correlation with the TH:TS/C ratios or with the AMLR pre- or postoperatively. It was found that the AMLR in 22 of 23 normal individuals was markedly inhibited by autologous T cells obtained from peripheral blood lymphocytes, exposed to 3,000 rad (Tx) and added as a third component to the cultures. In contrast, 13 of 13 kidney transplant recipients failed to exhibit this Tx AMLR inhibitory cell population. The "naturally occurring" T inhibitory cells, fractionated by an affinity column chromatography procedure into x-irradiated TH and TS/C subsets, inhibited the AMLR to the same extent as unseparated Tx cells. In cell interchange studies performed in four of five HLA identical donor-recipient pairs the Tx cells of the (normal) donor inhibited the recipient AMLR (immunosuppressed), but recipient Tx cells failed to inhibit the donor AMLR. Finally T cells, primed in AMLR and allogeneic MLR for 10 d were tested for AMLR or allogeneic MLR inhibitory activity. Allogeneic MLR primed x-irradiated cells, inhibited both the AMLR and allogeneic MLR while AMLR x-irradiated primed cells inhibited neither reaction. The Tx AMLR inhibitor found in normal peripheral blood, appears to be a cell that is highly sensitive to the effects of biologic or pharmacologic immunosuppressive agents.
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影响因子：
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作者：
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DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
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影响因子：
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作者：
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DOI：
10.1007/978-1-4684-3917-5_4
发表时间：
1981
期刊：
Contemporary topics in molecular immunology
影响因子：
--
作者：
Jacques Urbain;C. Wuilmart;P. Cazenave
通讯作者：
P. Cazenave