THE ADIPOSE CONVERSION OF 3T3 CELLS

3T3 细胞的脂肪转化

• 批准号: 3152312
• 负责人: HOWARD GREEN
• 金额: $ 25.12万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-03-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3152312
• 关键词: adipocytes; adipose tissue; cell differentiation; chromatin; cyclic AMP; fibroblasts; gene expression; genetic library; genetic mapping; genetic transcription; high performance liquid chromatography; isozymes; lipid biosynthesis; messenger RNA; molecular cloning; nucleic acid sequence; nucleosomes; radiotracer; regulatory gene; tissue /cell culture

When preadipose 3T3 cells arrest their growth and interact with an external adipogenic factor, they begin to convert to adipose cells. This is a faithful model of the formation of adipose cells in early developement. In the process of adipose conversion there is an extensive revision of the protein composition of the cytoplasm; this is necessary in order to provide the lipogenic and lipolytic enzymes and accesssory proteins for performance of the differentiated functions of the cell type. Using cDNA clones and ultimately genomic clones for some of the proteins participating in the differentiation, we will study the integrated or programmatic aspects of the adipose conversion, in order to learn how the cell coordinates the expression of this group of genes. Studies of transcription and mRNA formation in preadipose 3T3 cells, in converted 3T3 adipose cells and in cells of another 3T3 line unable to undergo conversion in response to adipogenic factor will enable us to define the order in which the genes respond. In particular, we will perform a kinetic analysis of these events during the course of adipose conversion. The relation of the order of expression of these genes to changes in chromatin structure will be explored. The expression of these genes will be studied in other tissues, such as liver and brain, whose program of differentiation overlaps the program in adipose differentiation. In this way we hope to learn how the expression of a given gene can be integrated into different programs in different cell types. These studies permit an approach to the study of obesity using cultured cells. Analysis of the molecular and cellular changes necessary for the formation of adipose cells from their precursors may be expected to shed light on the genesis of hyperplastic obesity.

当前脂肪3 T3细胞抑制其生长并与外部环境相互作用时， 成脂因子，它们开始转化为脂肪细胞。 这是一 在早期发育中脂肪细胞形成的可靠模型。 在 脂肪转化的过程中有一个广泛的修订 细胞质的蛋白质组成;这是必要的，以提供 脂肪生成酶和脂肪分解酶以及辅助蛋白质 细胞类型的分化功能。 使用cDNA克隆和 最后，基因组克隆的一些蛋白质参与 差异化，我们将研究的综合或方案方面， 脂肪转化，以了解细胞如何协调 这组基因的表达。 转录和mRNA研究 在前脂肪3 T3细胞中、在转化的3 T3脂肪细胞中和在转化的3 T3脂肪细胞中形成。 另一个3 T3系的细胞不能响应于 脂肪形成因子将使我们能够确定基因的顺序， 回答。 特别是，我们将对这些事件进行动力学分析 在脂肪转化的过程中。 的顺序关系 这些基因的表达对染色质结构的改变将是 探讨了 这些基因的表达将在其他组织中进行研究， 如肝脏和大脑，其分化程序与 脂肪分化的程序。 通过这种方式，我们希望了解 给定基因的表达可以整合到不同的程序中， 不同的细胞类型 这些研究允许一种方法来研究肥胖，使用培养的 细胞 分析细胞和分子的必要变化， 从其前体形成脂肪细胞可以预期脱落 关于增生性肥胖的起源