ESSENTIALITY OF DIETARY W-3 FATTY ACIDS IN PRIMATES

灵长类动物膳食 W-3 脂肪酸的重要性

• 批准号: 3151985
• 负责人: WILLIAM E CONNOR
• 金额: $ 11.28万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-07-01 至 1986-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3151985
• 关键词: adipose tissue; biopsy; blood chemistry; blood lipid; developmental nutrition; electroretinography; essential fatty acids; fatty acid metabolism; histology; learning; linolenate; newborn animals; nutrition related tag; pathogenic diet; pregnancy; psychological tests; skin; vision

The existence of a dietary requirement for fatty acids of the Omega3 or Alpha-linolenic acid family remains an unresolved issue in human nutrition. Like the Omega6 or linoleic acid family, Omega3 fatty acids cannot be synthesized by animals. The high content of Omega3 fatty acids in the retina and cerebral cortex suggests an important role in the function of neuronal and photoreceptor membranes. Previous studies of dietary deficiency of Omega3 fatty acids in rats demonstrated tissue depletion of Omega3 fatty acids, reduced electrophysiological responsiveness of the retina, and impaired learning ability. We propose to complete a long-term study of dietary deficiency of Omega3 fatty acids in nonhuman primates. Adult female rhesus monkeys are fed one of two semipurified diets for at least two months before mating and throughout pregnancy, and their infants then receive similar diets from birth to one year. The experimental diets, with fat supplied by safflower oil, have a very low content of Omega3 fatty acids; the control diets - identical except for the fat source, soybean oil - have a relatively high content of Omega3 fatty acids. Using biochemical, electrophysiological and behavioral techniques, we are monitoring the fatty acid composition of plasma and tissues and are testing the infants' visual function and learning ability. To date, levels of Omega3 fatty acids have fallen significantly in all plasma lipid classes of the experimental group's adult females and are nearly undetectable in the infants' plasma lipids. Skin and adipose tissue biopsies from the experimental infants also contain significantly reduced proportions of Omega3 fatty acids. Thus, the experimental diet has produced severe depletion of Omega3 fatty acids. A behavioral test of the infants' visual function demonstrated that the development of visual acuity was significantly impaired in the experimental group. The ability of the infants to learn a spatial reversal task, however, was not affected. Electroretinogram recordings and further tests of visual function are in progress. During the proposed two year renewal we plan to achieve the desired number of infants in both diet groups and to complete testing of visual function and behavior. At one year of age tissues, including retina and brain, will be examined for lipid and fatty acid content and for histological changes.

存在对欧米茄3或脂肪酸的饮食需求 α-亚麻酸家族在人类中仍然是一个尚未解决的问题 营养。像欧米茄6或亚油酸家族一样，欧米茄3脂肪酸 不能由动物合成。欧米茄3脂肪酸含量高 在视网膜和大脑皮层中起着重要作用 神经元膜和感光细胞膜的功能。以前的研究 大鼠膳食中Omega3脂肪酸的缺乏表现为组织 耗尽欧米茄3脂肪酸，减少电生理 视网膜的反应能力和学习能力受损。 我们建议完成对欧米茄3膳食不足的长期研究 非人灵长类动物中的脂肪酸。成年雌性恒河猴喂食一只 两种半纯净饲料交配前至少两个月 在整个怀孕期间，他们的婴儿接受类似的饮食 出生到一岁。试验性日粮，脂肪由红花提供 油，具有非常低的欧米茄3脂肪酸含量；对照饮食- 除了脂肪来源相同外，大豆油-都有相对较高的 欧米茄3脂肪酸的含量。使用生化、电生理和 行为技术，我们正在监测的脂肪酸组成 血浆和组织，正在测试婴儿的视觉功能和 学习能力。 到目前为止，所有人的欧米茄3脂肪酸水平都显著下降 试验组成年女性的血脂水平 在婴儿的血浆中几乎检测不到。皮肤和脂肪组织 实验婴儿的活组织检查中也含有显著减少的 欧米茄3脂肪酸的比例。因此，实验饮食有 导致欧米茄3脂肪酸严重枯竭。一种行为测试的方法 婴儿的视功能表明，视力的发展 在试验组中显著受损。美国人的能力 然而，婴儿学习空间反转任务并没有受到影响。 视网膜电波记录和视觉功能的进一步测试在 进步。 在拟议的两年续期期间，我们计划达到预期的数量 两个节食组的婴儿并完成视觉功能测试 和行为。在一岁时，包括视网膜和大脑在内的组织将 检查脂肪和脂肪酸含量以及组织学变化。